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Literature summary extracted from

  • Falara, V.; Alba, J.; Kant, M.; Schuurink, R.; Pichersky, E.
    Geranyllinalool synthases in Solanaceae and other angiosperms constitute an ancient branch of diterpene synthases involved in the synthesis of defensive compounds (2014), Plant Physiol., 166, 428-441 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.144 gene NaTPS46, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli Nicotiana attenuata
4.2.3.144 gene SlTPS46 encoding SlGLS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally His-tagged SlTPS46 in Escherichia coli Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.144 0.0187
-
 geranylgeranyl diphosphate pH and temperature not specified in the publication Solanum lycopersicum
4.2.3.144 0.0315
-
 geranylgeranyl diphosphate pH and temperature not specified in the publication Nicotiana attenuata
4.2.3.144 0.0637
-
 (2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.144 (2E,6E)-farnesyl diphosphate + H2O Solanum lycopersicum reaction of EC 4.2.3.48 (3S,6E)-nerolidol + diphosphate
-
 ?
4.2.3.144 geranylgeranyl diphosphate + H2O Solanum lycopersicum
-
 (6E,10E)-geranyllinalool + diphosphate
-
 ?
4.2.3.144 geranylgeranyl diphosphate + H2O Nicotiana attenuata
-
 (6E,10E)-geranyllinalool + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.144 Nicotiana attenuata A0A088DC07
-
-
4.2.3.144 Solanum lycopersicum K4BE78
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.144 recombinant N-terminally His-tagged SlTPS46 from Escherichia coli by nickel affinity chromatography Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.144 flower
-
 Solanum lycopersicum
-
4.2.3.144 fruit green and red Solanum lycopersicum
-
4.2.3.144 leaf
-
 Nicotiana attenuata
-
4.2.3.144 leaf expression is higher in young leaves compared to old ones Solanum lycopersicum
-
4.2.3.144 root
-
 Solanum lycopersicum
-
4.2.3.144 stem
-
 Solanum lycopersicum
-
4.2.3.144 trichome
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.144 (2E,6E)-farnesyl diphosphate + H2O reaction of EC 4.2.3.48 Solanum lycopersicum (3S,6E)-nerolidol + diphosphate
-
 ?
4.2.3.144 geranylgeranyl diphosphate + H2O
-
 Solanum lycopersicum (6E,10E)-geranyllinalool + diphosphate
-
 ?
4.2.3.144 geranylgeranyl diphosphate + H2O
-
 Nicotiana attenuata (6E,10E)-geranyllinalool + diphosphate
-
 ?
4.2.3.144 geranylgeranyl diphosphate + H2O preferred substrate Solanum lycopersicum (6E,10E)-geranyllinalool + diphosphate
-
 ?
4.2.3.144 additional information enzyme NaGLS is not active with geranyl diphosphate and farnesyl diphosphate, GC-MS analysis of compounds Nicotiana attenuata ?
-
 ?
4.2.3.144 additional information enzyme SlTPS46 shows no activity with geranyl diphospate, GC-MS analysis of compounds Solanum lycopersicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.144 101262489 gene name, UniProt Solanum lycopersicum
4.2.3.144 geranyllinalool synthase
-
 Solanum lycopersicum
4.2.3.144 geranyllinalool synthase
-
 Nicotiana attenuata
4.2.3.144 GES gene name, UniProt Nicotiana attenuata
4.2.3.144 GLS
-
 Solanum lycopersicum
4.2.3.144 GLS
-
 Nicotiana attenuata
4.2.3.144 More cf. EC 4.2.3.48 Solanum lycopersicum
4.2.3.144 NaGLS
-
 Nicotiana attenuata
4.2.3.144 SlGLS
-
 Solanum lycopersicum
4.2.3.144 SlTPS46
-
 Solanum lycopersicum
4.2.3.144 TPS-e/f
-
 Solanum lycopersicum
4.2.3.144 TPS-e/f
-
 Nicotiana attenuata
4.2.3.144 TPS46
-
 Solanum lycopersicum
4.2.3.144 TPS46
-
 Nicotiana attenuata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.144 0.43
-
 (2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Solanum lycopersicum
4.2.3.144 6.85
-
 geranylgeranyl diphosphate pH and temperature not specified in the publication Solanum lycopersicum
4.2.3.144 7.53
-
 geranylgeranyl diphosphate pH and temperature not specified in the publication Nicotiana attenuata

Expression

EC Number Organism Comment Expression
4.2.3.144 Solanum lycopersicum suppression of the enzyme by methyl salicylate down
4.2.3.144 Solanum lycopersicum no induction of the enzyme by the defense-suppressing mite line Tetranycus evansi Vicosa-1 additional information
4.2.3.144 Nicotiana attenuata NaGLS is expressed constitutively, but the gene NaTPS46 can be induced in leaves with methyl jasmonate up
4.2.3.144 Solanum lycopersicum TPS46 shows induction by alamethicin and methyljasmonate treatment, induction by defense-inducing spider mite line Tetranycus urticae Santpoort-2 up

General Information

EC Number General Information Comment Organism
4.2.3.144 evolution SlGLS, NaGLS, AtGLSs, and several other GLSs come from different eudicot families and constitute a separate branch of the TPS-e/f clade that diverged from kaurene synthases, also in the TPS-e/f clade, before the gymnosperm-angiosperm split. The early divergence of this branch and the GLS activity of genes in this branch in diverse eudicot families suggest that GLS activity encoded by these genes predates the angiosperm-gymnosperm split Solanum lycopersicum
4.2.3.144 evolution SlGLS, NaGLS, AtGLSs, and several other GLSs come from different eudicot families and constitute a separate branch of the TPS-e/f clade that diverged from kaurene synthases, also in the TPS-e/f clade, before the gymnosperm-angiosperm split. The early divergence of this branch and the GLS activity of genes in this branch in diverse eudicot families suggest that GLS activity encoded by these genes predates the angiosperm-gymnosperm split Nicotiana attenuata
4.2.3.144 metabolism the terpene synthase (TPS) catalyzes a two-step reaction in which carbocation formation of the C20 precursor geranylgeranyl diphosphate (GGPP) is followed by an allylic rearrangement that results in the production of the tertiary alcohol geranyllinalool, which is the precursor for biosynthesis of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene, TMTT Solanum lycopersicum
4.2.3.144 metabolism the terpene synthase (TPS) catalyzes a two-step reaction in which carbocation formation of the C20 precursor geranylgeranyl diphosphate (GGPP) is followed by an allylic rearrangement that results in the production of the tertiary alcohol geranyllinalool, which is the precursor for biosynthesis of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene, TMTT Nicotiana attenuata
4.2.3.144 physiological function GC-MS analysis of terpene volatiles induced by alamethicin treatment, overview Solanum lycopersicum
4.2.3.144 physiological function GC-MS analysis of terpene volatiles induced by alamethicin treatment, overview Nicotiana attenuata

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.144 6.75
-
 (2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Solanum lycopersicum
4.2.3.144 239.05
-
 geranylgeranyl diphosphate pH and temperature not specified in the publication Nicotiana attenuata
4.2.3.144 366.3
-
 geranylgeranyl diphosphate pH and temperature not specified in the publication Solanum lycopersicum