EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.120 | gene CYP716A47, recombinant expression under control of 35S CaMV promoter in leaves of tobacco via Agrobacterium tumefaciens GV3101 transformation method, RT-PCR expression analysis | Panax ginseng |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.120 | endoplasmic reticulum | - |
Panax ginseng | 5783 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.120 | dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2 | Panax ginseng | hydroxylation of dammarenediol-II at C-12 | protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.120 | Panax ginseng | H2DH16 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.120 | cell suspension culture | - |
Panax ginseng | - |
1.14.14.120 | root | - |
Panax ginseng | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.120 | dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation of dammarenediol-II at C-12 | Panax ginseng | protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.120 | CYP716A47 | - |
Panax ginseng |
1.14.14.120 | PPDS | - |
Panax ginseng |
1.14.14.120 | protopanaxadiol synthase | - |
Panax ginseng |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.120 | cytochrome P450 | - |
Panax ginseng | |
1.14.14.120 | NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Panax ginseng |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.14.120 | Panax ginseng | auxin treatment, particularly 2,4-dichlorophenoxyacetic acid, strongly enhances the transcription of the HMG-Co reductase (HMGR) and squalene epoxidase genes and thus the production of dammarenediol-II and protopanaxadiol (PPD) | additional information |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.120 | metabolism | the enzyme is part of the biosynthetic pathway for ginsenoside production in Panax ginseng, overview | Panax ginseng |
1.14.14.120 | physiological function | protopanaxadiol (PPD) is an aglycone of dammarene-type ginsenoside and has a wide range of pharmacological activities and high medicinal values. It is synthesized via the hydroxylation of dammarenediol-II (DD) by CYP716A47 enzyme | Panax ginseng |