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Literature summary extracted from
- Transcriptomic analysis of Kalopanax septemlobus and characterization of KsBAS, CYP716A94, and CYP72A397 genes involved in hederagenin saponin biosynthesis (2017), Plant Cell Physiol., 59, 319-330 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.126 | gene CYP716A94, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain WAT21 microsomes, coexpression with Arabidopsis thaliana NADPH-CYP reductase leads to production of oleanolic acid, mass spectrometric analysis | Kalopanax septemlobus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.126 | membrane | a transmembrane enzyme | Kalopanax septemlobus | 16020 | - |
| 1.14.14.126 | microsome | - |
Kalopanax septemlobus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.126 | Fe2+ | bound in heme | Kalopanax septemlobus |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.126 | beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Kalopanax septemlobus | - |
oleanolate + [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.126 | Kalopanax septemlobus | A0A0S2II38 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.126 | beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Kalopanax septemlobus | oleanolate + [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.126 | beta-amyrin 28-oxidase | - |
Kalopanax septemlobus |
| 1.14.14.126 | CYP716A94 | - |
Kalopanax septemlobus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.126 | 30 | - |
in vivo assay at | Kalopanax septemlobus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.126 | cytochrome P450 | - |
Kalopanax septemlobus | |
| 1.14.14.126 | heme | - |
Kalopanax septemlobus | |
| 1.14.14.126 | NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Kalopanax septemlobus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.126 | metabolism | the enzyme catalyzes a step in the putative saponin biosynthetic pathway from 2,3-oxidosqualene to hederagenin saponin, overview. Two enzymes (beta-amyrin synthase and beta-amyrin 28-oxidase) are essential for oleanane-type saponin biosynthesis from 2,3-oxidosqualene | Kalopanax septemlobus |
| 1.14.14.126 | physiological function | CYP716A94 is a beta-amyrin 28-oxidase for the production of oleanolic acid | Kalopanax septemlobus |
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Release 2023.1 (January 2023)
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