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Literature summary extracted from
- Cytochrome P450 monooxygenase CYP716A141 is a unique beta-amyrin C-16beta oxidase involved in triterpenoid saponin biosynthesis in Platycodon grandiflorus (2017), Plant Cell Physiol., 58, 874-884 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.63 | expression in Saccharomyces cerevisiae | Platycodon grandiflorus |
| 1.14.14.115 | gene CYP716A141, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels | Platycodon grandiflorus |
| 1.14.14.126 | gene CYP716A140v2, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels | Platycodon grandiflorus |
| 1.14.14.126 | gene CYP716A141, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels | Platycodon grandiflorus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.115 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | Platycodon grandiflorus | hydroxylation at C-16beta, main activity of enzyme CYP716A141 | maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |  |
| 1.14.14.115 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | Platycodon grandiflorus | hydroxylation at C-28, reaction of EC 1.14.13.201 | oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.115 | maniladiol + [reduced NADPH-hemoprotein reductase] + O2 | Platycodon grandiflorus | hydroxylation at C-28 | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.115 | oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2 | Platycodon grandiflorus | hydroxylation at C-16beta | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.126 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | Platycodon grandiflorus | hydroxylation at C-16beta | maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.126 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | Platycodon grandiflorus | hydroxylation at C-28 | oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.126 | maniladiol + [reduced NADPH-hemoprotein reductase] + O2 | Platycodon grandiflorus | hydroxylation at C-28 | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.126 | oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2 | Platycodon grandiflorus | hydroxylation at C-16beta, reaction of EC 1.14.13.173 | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.63 | Platycodon grandiflorus | A0A1I9Q5Z0 | - |
- |
| 1.14.14.115 | Platycodon grandiflorus | A0A1I9Q5Z0 | - |
- |
| 1.14.14.126 | Platycodon grandiflorus | A0A1I9Q5Z0 | - |
- |
| 1.14.14.126 | Platycodon grandiflorus | A0A1V1FHP8 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.63 | root | - |
Platycodon grandiflorus | - |
| 1.14.14.115 | leaf | - |
Platycodon grandiflorus | - |
| 1.14.14.115 | additional information | transcriptome analysis in different tissues using RNA sequencing technology, overview | Platycodon grandiflorus | - |
| 1.14.14.115 | petal | - |
Platycodon grandiflorus | - |
| 1.14.14.115 | root | the enzyme is highly expressed in roots | Platycodon grandiflorus | - |
| 1.14.14.126 | leaf | - |
Platycodon grandiflorus | - |
| 1.14.14.126 | additional information | transcriptome analysis in different tissues using RNA sequencing technology, overview | Platycodon grandiflorus | - |
| 1.14.14.126 | petal | - |
Platycodon grandiflorus | - |
| 1.14.14.126 | root | the enzyme is highly expressed in roots | Platycodon grandiflorus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.63 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Platycodon grandiflorus | maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.63 | additional information | enzyme catalyzes the hydroxylation of beta-amyrin at C-16beta, plus further oxidation at C-28 to cochalic acid as a minor reaction | Platycodon grandiflorus | ? | - |
? | |
| 1.14.14.115 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at C-16beta, main activity of enzyme CYP716A141 | Platycodon grandiflorus | maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.115 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at C-28, reaction of EC 1.14.13.201 | Platycodon grandiflorus | oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.115 | maniladiol + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at C-28 | Platycodon grandiflorus | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.115 | additional information | CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry | Platycodon grandiflorus | ? | - |
? | |
| 1.14.14.115 | oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at C-16beta | Platycodon grandiflorus | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.126 | beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | overall reaction | Platycodon grandiflorus | oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
| 1.14.14.126 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Platycodon grandiflorus | erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.126 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at C-16beta | Platycodon grandiflorus | maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.126 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at C-28 | Platycodon grandiflorus | oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.126 | erythrodiol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Platycodon grandiflorus | oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| 1.14.14.126 | maniladiol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Platycodon grandiflorus | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.126 | maniladiol + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at C-28 | Platycodon grandiflorus | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.126 | additional information | CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry | Platycodon grandiflorus | ? | - |
? | |
| 1.14.14.126 | additional information | hydroxylation of beta-amyrin at C-16beta is the main activity of enzyme CYP716A141, but CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry. The enzyme hydroxylates oleanolic acid, beta-amyrin, and maniladiol | Platycodon grandiflorus | ? | - |
? | |
| 1.14.14.126 | oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at C-16beta, reaction of EC 1.14.13.173 | Platycodon grandiflorus | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.126 | oleanolic aldehyde + [reduced NADPH-hemoprotein reductase] + O2 | - |
Platycodon grandiflorus | oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.63 | Beta-amyrin 16-oxidase | - |
Platycodon grandiflorus |
| 1.14.14.63 | CYP716A141 | - |
Platycodon grandiflorus |
| 1.14.14.115 | beta-amyrin C-16beta oxidase | - |
Platycodon grandiflorus |
| 1.14.14.115 | C-16beta oxidase | - |
Platycodon grandiflorus |
| 1.14.14.115 | CYP716A141 | - |
Platycodon grandiflorus |
| 1.14.14.115 | More | cf. EC 1.14.13.201, beta-amyrin C-28 oxidase | Platycodon grandiflorus |
| 1.14.14.126 | beta-amyrin 28-oxidase | - |
Platycodon grandiflorus |
| 1.14.14.126 | CYP716A140v2 | - |
Platycodon grandiflorus |
| 1.14.14.126 | CYP716A141 | - |
Platycodon grandiflorus |
| 1.14.14.126 | More | cf. EC 1.14.13.173, beta-amyrin C-16beta oxidase | Platycodon grandiflorus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.115 | 30 | - |
in vivo assay at | Platycodon grandiflorus |
| 1.14.14.126 | 30 | - |
in vivo assay at | Platycodon grandiflorus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.63 | cytochrome P450 | - |
Platycodon grandiflorus | |
| 1.14.14.115 | cytochrome P450 | - |
Platycodon grandiflorus | |
| 1.14.14.115 | NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Platycodon grandiflorus | |
| 1.14.14.126 | cytochrome P450 | - |
Platycodon grandiflorus | |
| 1.14.14.126 | NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Platycodon grandiflorus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.115 | evolution | the enzyme belongs to the CYP716A subfamily of cytochrome P450 monooxygenases | Platycodon grandiflorus |
| 1.14.14.126 | evolution | the enzyme belongs to the CYP716A subfamily of cytochrome P450 monooxygenases | Platycodon grandiflorus |
| 1.14.14.126 | physiological function | CYP716A140v2, catalyzed a three-step oxidation reaction at C-28 on beta-amyrin to produce oleanolic acid, a reaction performed by CYP716A subfamily P450s in a variety of plant species | Platycodon grandiflorus |
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