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Literature summary extracted from

  • Dai, L.; Liu, C.; Zhu, Y.; Zhang, J.; Men, Y.; Zeng, Y.; Sun, Y.
    Functional characterization of cucurbitadienol synthase and triterpene glycosyltransferase involved in biosynthesis of mogrosides from Siraitia grosvenorii (2015), Plant Cell Physiol., 56, 1172-1182 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.350 expressed in Escherichia coli Rosetta-gami (DE3) cells Siraitia grosvenorii
5.4.99.33 gene CBS, DNA and amino acid sequence determination and analysis, digital gene expression (DGE) profile analysis, sequence comparisons and phylogenetic analysis of codon-optimized gene, SgCbQ is functionally expressed in the lanosterol synthase-deficient Saccharomyces cerevisiae strain GIL77 leading to accumulation of cucurbitadienol as the sole product Siraitia grosvenorii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.350 0.0414
-
 mogrol at pH 7.2 and 30°C Siraitia grosvenorii
2.4.1.350 0.0547
-
 naringenin at pH 7.2 and 30°C Siraitia grosvenorii
2.4.1.350 0.0582
-
 quercetin at pH 7.2 and 30°C Siraitia grosvenorii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.350 UDP-alpha-D-glucose + mogrol Siraitia grosvenorii
-
 mogroside IE + UDP
-
 ?
5.4.99.33 (3S)-2,3-epoxy-2,3-dihydrosqualene Siraitia grosvenorii
-
 cucurbitadienol
-
 ?
5.4.99.33 additional information Siraitia grosvenorii GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.350 Siraitia grosvenorii
-
-
-
5.4.99.33 Siraitia grosvenorii K7NBZ9 cultivated in Guangxi Province of China
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.350 Ni-NTA agarose column chromatography Siraitia grosvenorii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.350 fruit
-
 Siraitia grosvenorii
-
5.4.99.33 fruit expression profiles of SgCbQ gene during the three important stages of fruit development Siraitia grosvenorii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.350 UDP-alpha-D-glucose + mogrol
-
 Siraitia grosvenorii mogroside IE + UDP
-
 ?
2.4.1.350 UDP-alpha-D-glucose + naringenin
-
 Siraitia grosvenorii ?
-
 ?
2.4.1.350 UDP-alpha-D-glucose + quercetin
-
 Siraitia grosvenorii ?
-
 ?
5.4.99.33 (3S)-2,3-epoxy-2,3-dihydrosqualene
-
 Siraitia grosvenorii cucurbitadienol
-
 ?
5.4.99.33 additional information GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol Siraitia grosvenorii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.350 ? x * 52000, SDS-PAGE Siraitia grosvenorii

Synonyms

EC Number Synonyms Comment Organism
2.4.1.350 UGT74AC1
-
 Siraitia grosvenorii
5.4.99.33 CBS
-
 Siraitia grosvenorii
5.4.99.33 SgCbQ
-
 Siraitia grosvenorii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.350 0.000411
-
 mogrol at pH 7.2 and 30°C Siraitia grosvenorii
2.4.1.350 0.41
-
 quercetin at pH 7.2 and 30°C Siraitia grosvenorii
2.4.1.350 0.45
-
 naringenin at pH 7.2 and 30°C Siraitia grosvenorii

General Information

EC Number General Information Comment Organism
5.4.99.33 evolution SgCbQ clusters with the other three functionally characterized cucurbitadienol synthases and forms a new subfamily that lies between the branches of cycloartenol synthase and lanosterol synthase, non-rooted phylogenetic tree analysis, overview Siraitia grosvenorii
5.4.99.33 metabolism cucurbitadienol synthase and triterpene glycosyltransferase are involved in biosynthesis of mogrosides in Siraitia grosvenorii. Mogrosides, the major bioactive components isolated from the fruits of Siraitia grosvenorii, are a family of cucurbitane-type tetracyclic triterpenoid saponins that are used worldwide as high-potency sweeteners and possess a variety of notable pharmacological activities. Mogrosides are synthesized from 2,3-oxidosqualene via a series of reactions catalyzed by cucurbitadienol synthase (CbQ), Cyt P450s (P450s), and UDP glycosyltransferases (UGTs) in vivo, proposed biosynthetic pathway of mogrosides, overview Siraitia grosvenorii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.350 0.00994
-
 mogrol at pH 7.2 and 30°C Siraitia grosvenorii
2.4.1.350 6.88
-
 quercetin at pH 7.2 and 30°C Siraitia grosvenorii
2.4.1.350 8.23
-
 naringenin at pH 7.2 and 30°C Siraitia grosvenorii