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Literature summary extracted from
- Evolution of a new chlorophyll metabolic pathway driven by the dynamic changes in enzyme promiscuous activity (2014), Plant Cell Physiol., 55, 593-603 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.3.1.75 | gene DVR, phylogenetic analysis | Arabidopsis thaliana |
| 1.3.1.75 | gene DVR, phylogenetic analysis, recombinant expression of NADPH-dependent DVR in Escherichia coli | Phaeodactylum tricornutum |
| 1.3.7.13 | gene slr1923, phylogenetic analysis | Synechocystis sp. PCC 6803 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.75 | 3,8-divinyl chlorophyll a + NADPH + H+ | Phaeodactylum tricornutum | very low activity | chlorophyll a + NADP+ | - |
? |  |
| 1.3.1.75 | 3,8-divinyl chlorophyllide a + NADPH + H+ | Phaeodactylum tricornutum | - |
chlorophyllide a + NADP+ | - |
? | |
| 1.3.1.75 | 3,8-divinyl chlorophyllide a + NADPH + H+ | Arabidopsis thaliana | low activity | chlorophyllide a + NADP+ | - |
? | |
| 1.3.7.13 | 3,8-divinyl chlorophyll a + reduced ferredoxin [iron-sulfur] cluster + H+ | Synechocystis sp. PCC 6803 | - |
chlorophyll a + oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
| 1.3.7.13 | 3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ | Synechocystis sp. PCC 6803 | - |
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
| 1.3.7.13 | 3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ | Synechocystis sp. PCC 6803 | - |
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
| 1.3.7.13 | additional information | Synechocystis sp. PCC 6803 | cyanobacterial 3,8-divinyl chlorophyllide reductase (DVR) is a Chl biosynthetic enzyme that has many promiscuous activities besides the 3,8-divinyl chlorophyllide reductase activity, e.g. 7-hydroxymethyl chlorophyll a reductase activity. The Chl c2 to Chl c1 conversion is existent in cyanobacteria as a promiscuous activity of F-DVR. Chl c1 is synthesized by the reduction of the 8-vinyl group of Chl c2 or Chl c1 is synthesized from monovinyl-protochlorophyllide by the dehydrogenase | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.75 | Arabidopsis thaliana | Q1H537 | - |
- |
| 1.3.1.75 | Phaeodactylum tricornutum | - |
- |
- |
| 1.3.7.13 | Synechocystis sp. PCC 6803 | P74473 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.75 | 3,8-divinyl chlorophyll a + NADPH + H+ | very low activity | Phaeodactylum tricornutum | chlorophyll a + NADP+ | - |
? | |
| 1.3.1.75 | 3,8-divinyl chlorophyll a + NADPH + H+ | very low activity | Arabidopsis thaliana | chlorophyll a + NADP+ | - |
? | |
| 1.3.1.75 | 3,8-divinyl chlorophyllide a + NADPH + H+ | - |
Phaeodactylum tricornutum | chlorophyllide a + NADP+ | - |
? | |
| 1.3.1.75 | 3,8-divinyl chlorophyllide a + NADPH + H+ | - |
Arabidopsis thaliana | chlorophyllide a + NADP+ | - |
? | |
| 1.3.1.75 | 3,8-divinyl chlorophyllide a + NADPH + H+ | low activity | Arabidopsis thaliana | chlorophyllide a + NADP+ | - |
? | |
| 1.3.1.75 | additional information | substrate specificity, overview. 3,8-Divinylprotochlorophyllide is a poor substrate, N-DVR has a high specificity for 3,8-divinyl chlorophyllide a | Arabidopsis thaliana | ? | - |
? | |
| 1.3.1.75 | additional information | substrate specificity, overview. Diatom N-DVR cannot convert 3,8-divinyl-protochlorophyllide a to 3-vinyl-protochlorophyllide a, but diatom N-DVR can efficiently utilize both 3,8-divinyl-chlorophyll a and 3,8-divinyl-chlorophyllide a, different from other N-DVRs, though in vitro, diatom N-DVR does not convert Chl c2 to Chl c1. No ferredoxin-dependent F-DVR activity, EC 1.3.7.13 | Phaeodactylum tricornutum | ? | - |
? | |
| 1.3.7.13 | 3,8-divinyl chlorophyll a + reduced ferredoxin [iron-sulfur] cluster + H+ | - |
Synechocystis sp. PCC 6803 | chlorophyll a + oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
| 1.3.7.13 | 3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ | - |
Synechocystis sp. PCC 6803 | chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
| 1.3.7.13 | 3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ | - |
Synechocystis sp. PCC 6803 | protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
| 1.3.7.13 | additional information | cyanobacterial 3,8-divinyl chlorophyllide reductase (DVR) is a Chl biosynthetic enzyme that has many promiscuous activities besides the 3,8-divinyl chlorophyllide reductase activity, e.g. 7-hydroxymethyl chlorophyll a reductase activity. The Chl c2 to Chl c1 conversion is existent in cyanobacteria as a promiscuous activity of F-DVR. Chl c1 is synthesized by the reduction of the 8-vinyl group of Chl c2 or Chl c1 is synthesized from monovinyl-protochlorophyllide by the dehydrogenase | Synechocystis sp. PCC 6803 | ? | - |
? | |
| 1.3.7.13 | additional information | substrate specificity, overview. Cyanobacterial F-DVR exhibits a broad substrate specificity and converts 3,8-divinylprotochlorophyllide a, 3,8-divinyl chlorophyllide a, and 3,8-divinyl chlorophyll a to the respective monovinyl molecules. When 7-hydroxymethyl chlorophyllide (HMChlide) a is incubated with F-DVR, chlorophyllide a is generated | Synechocystis sp. PCC 6803 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.3.1.75 | 3,8-divinyl chlorophyllide reductase | - |
Phaeodactylum tricornutum |
| 1.3.1.75 | 3,8-divinyl chlorophyllide reductase | - |
Arabidopsis thaliana |
| 1.3.1.75 | AtN-DVR | - |
Arabidopsis thaliana |
| 1.3.1.75 | DVR | - |
Phaeodactylum tricornutum |
| 1.3.1.75 | DVR | - |
Arabidopsis thaliana |
| 1.3.1.75 | N-DVR | - |
Phaeodactylum tricornutum |
| 1.3.1.75 | N-DVR | - |
Arabidopsis thaliana |
| 1.3.1.75 | NADPH-dependent DVR | - |
Phaeodactylum tricornutum |
| 1.3.1.75 | NADPH-dependent DVR | - |
Arabidopsis thaliana |
| 1.3.1.75 | PhaeN-DVR | - |
Phaeodactylum tricornutum |
| 1.3.7.13 | 3,8-divinyl chlorophyllide reductase | - |
Synechocystis sp. PCC 6803 |
| 1.3.7.13 | DVR | - |
Synechocystis sp. PCC 6803 |
| 1.3.7.13 | F-DVR | - |
Synechocystis sp. PCC 6803 |
| 1.3.7.13 | Fd-dependent DVR | - |
Synechocystis sp. PCC 6803 |
| 1.3.7.13 | Slr1923 | - |
Synechocystis sp. PCC 6803 |
| 1.3.7.13 | Slr1923 potein | - |
Synechocystis sp. PCC 6803 |
| 1.3.7.13 | SyF-DVR | - |
Synechocystis sp. PCC 6803 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.75 | 25 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.75 | 7.5 | - |
assay at | Arabidopsis thaliana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.75 | NADPH | dependent on | Phaeodactylum tricornutum | |
| 1.3.1.75 | NADPH | dependent on | Arabidopsis thaliana | |
| 1.3.7.13 | ferredoxin [iron-sulfur] cluster | dependent on | Synechocystis sp. PCC 6803 | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.3.1.75 | evolution | either F-DVR or N-DVR is found in most photosynthetic organisms, yet both F-DVR and N-DVR exist in the genome of diatoms that contain Chl a and Chl c1 | Arabidopsis thaliana |
| 1.3.1.75 | evolution | either F-DVR or N-DVR is found in most photosynthetic organisms, yet both F-DVR and N-DVR exist in the genome of diatoms that contain Chl a and Chl c1. Diatom N-DVR has evolved to function in Chl a biosynthesis, and diatom F-DVR is responsible for the biosynthesis of Chl c1 from Chl c2 | Phaeodactylum tricornutum |
| 1.3.1.75 | metabolism | the enzyme is involved the chlorophyll biosynthetic pathways of oxygenic photosynthetic organisms. At the later steps of chlorophyll biosynthesis, 3,8-divinyl-chlorophyllide (Chlide) a is converted to monovinyl (MV)-Chlide a by DVR | Phaeodactylum tricornutum |
| 1.3.1.75 | metabolism | the enzyme is involved the chlorophyll biosynthetic pathways of oxygenic photosynthetic organisms. At the later steps of chlorophyll biosynthesis, 3,8-divinyl-chlorophyllide (Chlide) a is converted to monovinyl (MV)-Chlide a by DVR | Arabidopsis thaliana |
| 1.3.7.13 | evolution | either F-DVR or N-DVR is found in most photosynthetic organisms, yet both F-DVR and N-DVR exist in the genome of diatoms that contain Chl a and Chl c1 | Synechocystis sp. PCC 6803 |
| 1.3.7.13 | metabolism | the enzyme is involved the chlorophyll biosynthetic pathways of oxygenic photosynthetic organisms. At the later steps of chlorophyll biosynthesis, 3,8-divinyl-chlorophyllide (Chlide) a is converted to monovinyl (MV)-Chlide a by DVR. Involvement of DVR in Chl c biosynthesis | Synechocystis sp. PCC 6803 |
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