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Literature summary extracted from

  • Hackenberg, C.; Kern, R.; Huege, J.; Stal, L.J.; Tsuji, Y.; Kopka, J.; Shiraiwa, Y.; Bauwe, H.; Hagemann, M.
    Cyanobacterial lactate oxidases serve as essential partners in N2 fixation and evolved into photorespiratory glycolate oxidases in plants (2011), Plant Cell, 23, 2978-2990 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.2 F212V change in the active site to that of Arabidopsis thaliana glycolate oxidase 2, 25fold decrease in the L-lactate oxidase/glycolate oxidase activity ratio Nostoc sp. PCC 7120 = FACHB-418
1.1.3.2 L112W change in the active site to that of Arabidopsis thaliana glycolate oxidase 2, 2fold decrease in the L-lactate oxidase/glycolate oxidase activity ratio Nostoc sp. PCC 7120 = FACHB-418
1.1.3.2 M82T change in the active site to that of Arabidopsis thaliana glycolate oxidase 2, 10fold decrease in the L-lactate oxidase/glycolate oxidase activity ratio Nostoc sp. PCC 7120 = FACHB-418
1.1.3.2 M82T/L112W/F212V change in the active site to that of Arabidopsis thaliana glycolate oxidase 2, reverse the L-lactate oxidase/glycolate oxidase activity ratio Nostoc sp. PCC 7120 = FACHB-418

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.2 0.039
-
L-lactate pH 8.0, 30°C Nostoc sp. PCC 7120 = FACHB-418
1.1.3.2 0.081
-
L-lactate pH 8.0, 30°C Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.2 Chlamydomonas reinhardtii F8WQN2 cf. EC 1.1.3.1, enzyme shows high L-lactate oxidase and low glycolate oxidase activities
-
1.1.3.2 Nostoc sp. PCC 7120 = FACHB-418 Q8Z0C8 cf. EC 1.1.3.1, enzyme shows high L-lactate oxidase and low glycolate oxidase activities
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.2 glycerate + O2 5.9% of the activity with L-lactate Nostoc sp. PCC 7120 = FACHB-418 hydroxypyruvate + H2O2
-
?
1.1.3.2 glyoxylate + O2 15.3% of the activity with L-lactate Nostoc sp. PCC 7120 = FACHB-418 oxalate + H2O2
-
?
1.1.3.2 L-lactate + O2
-
Nostoc sp. PCC 7120 = FACHB-418 pyruvate + H2O2
-
?
1.1.3.2 L-lactate + O2
-
Chlamydomonas reinhardtii pyruvate + H2O2
-
?
1.1.3.2 additional information no substrate: D-lactate. Poor substrate: glycolate Nostoc sp. PCC 7120 = FACHB-418 ?
-
?
1.1.3.2 additional information no substrate: D-lactate. Poor substrate: glycolate Chlamydomonas reinhardtii ?
-
?

General Information

EC Number General Information Comment Organism
1.1.3.2 physiological function overexpression decreases the accumulation of toxic glycolate in a cyanobacterial photorespiratory mutant and restores its ability to grow in air. A lactate oxidase-deficient Nostoc sp. mutant grows normally in nitrate-containing medium but dies under N2-fixing conditions. The enzyme may primarily serve as an O2-scavenging enzyme to protect nitrogenase in extant N2-fixing cyanobacteria Nostoc sp. PCC 7120 = FACHB-418