EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.87 | gene ifs1, full-length GmIFS1 cDNA is isolated through RT-PCR of total RNA of soybean seedling, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana tabacum cv. Petit Havana via Agrobacterium tumefaciens strain LBA4404 transformation method leading to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. real-time PCR and RT-PCR enzyme expression analysis | Glycine max |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.87 | additional information | oestrogen-deficient (ovariectomized, Ovx) mice fed with leaf extract from transgenic plant co-expressing AtMYB12 and GmIFS1 but not wild-type extract exhibit significant conservation of trabecular microarchitecture, reduced osteoclast number and expression of osteoclastogenic genes, higher total serum antioxidant levels, and increased uterine oestrogenicity compared with Ovx mice treated with vehicle (control). The simultaneous coexpression of AtMYB12 and GmIFS1 in tobacco leads to the accumulation of substantial amount of genistein in addition to flavonols | Glycine max |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.87 | liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 | Glycine max | - |
2,4',7-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.87 | Glycine max | Q9M6D6 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.87 | liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Glycine max | 2,4',7-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.87 | GmIFS1 | - |
Glycine max |
1.14.14.87 | isoflavone synthase | - |
Glycine max |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.87 | NADPH | - |
Glycine max |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.14.87 | Glycine max | gene ifs1, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana benthamiana leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity | up |