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Literature summary extracted from

  • Pandey, A.; Misra, P.; Khan, M.; Swarnkar, G.; Tewari, M.; Bhambhani, S.; Trivedi, R.; Chattopadhyay, N.; Trivedi, P.
    Co-expression of Arabidopsis transcription factor, AtMYB12, and soybean isoflavone synthase, GmIFS1, genes in tobacco leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity (2014), Plant Biotechnol. J., 12, 69-80 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.87 gene ifs1, full-length GmIFS1 cDNA is isolated through RT-PCR of total RNA of soybean seedling, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana tabacum cv. Petit Havana via Agrobacterium tumefaciens strain LBA4404 transformation method leading to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. real-time PCR and RT-PCR enzyme expression analysis Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.87 additional information oestrogen-deficient (ovariectomized, Ovx) mice fed with leaf extract from transgenic plant co-expressing AtMYB12 and GmIFS1 but not wild-type extract exhibit significant conservation of trabecular microarchitecture, reduced osteoclast number and expression of osteoclastogenic genes, higher total serum antioxidant levels, and increased uterine oestrogenicity compared with Ovx mice treated with vehicle (control). The simultaneous coexpression of AtMYB12 and GmIFS1 in tobacco leads to the accumulation of substantial amount of genistein in addition to flavonols Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.87 liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 Glycine max
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2,4',7-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.87 Glycine max Q9M6D6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.87 liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2
-
Glycine max 2,4',7-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.87 GmIFS1
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Glycine max
1.14.14.87 isoflavone synthase
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Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.87 NADPH
-
Glycine max

Expression

EC Number Organism Comment Expression
1.14.14.87 Glycine max gene ifs1, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana benthamiana leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity up