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Literature summary extracted from

  • Peng, C.; Liang, X.; Liu, E.E.; Zhang, J.J.; Peng, X.X.
    The oxalyl-CoA synthetase-regulated oxalate and its distinct effects on resistance to bacterial blight and aluminium toxicity in rice (2017), Plant Biol., 19, 345-353 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.8 expressed in Escherichia coli BL21(DE3) cells Oryza sativa

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.8 C219G the mutant shows 5.6% activity compared to the wild type enzyme Oryza sativa
6.2.1.8 C287G the mutant shows 25.4% activity compared to the wild type enzyme Oryza sativa
6.2.1.8 C363G the mutant shows 7.2% activity compared to the wild type enzyme Oryza sativa
6.2.1.8 C454G the mutant shows 44.5% activity compared to the wild type enzyme Oryza sativa
6.2.1.8 C473G the mutant shows 42.9% activity compared to the wild type enzyme Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.8 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide almost complete inhibition at 5 mM Oryza sativa
6.2.1.8 5,5'-dithiobis-(2-nitrobenzoic acid) less than 10% residual activity at 0.1 mM Oryza sativa
6.2.1.8 N-ethylmaleimide complete inhibition in Tris-HCl at 0.1 mM Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.8 1.73
-
 oxalate wild type enzyme, at pH 7.5 and 37°C Oryza sativa
6.2.1.8 2.62
-
 oxalate mutant enzyme C473G, at pH 7.5 and 37°C Oryza sativa
6.2.1.8 3.82
-
 oxalate mutant enzyme C219G, at pH 7.5 and 37°C Oryza sativa
6.2.1.8 4.64
-
 oxalate mutant enzyme C363G, at pH 7.5 and 37°C Oryza sativa
6.2.1.8 44.5
-
 oxalate mutant enzyme C454G, at pH 7.5 and 37°C Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.8 cytosol
-
 Oryza sativa 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.8 ATP + oxalate + CoA Oryza sativa the enzyme is highly specific for oxalate AMP + diphosphate + oxalyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.8 Oryza sativa
-
 cultivar Zhonghua 11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.8 nickel chelating affinity column chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.8 leaf epidermis
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.8 ATP + oxalate + CoA the enzyme is highly specific for oxalate Oryza sativa AMP + diphosphate + oxalyl-CoA
-
 ?
6.2.1.8 additional information no activity with malonate, succinate, malate, acetate, formate, lactate, glycolate, and glyoxylate Oryza sativa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.8 AAE3
-
 Oryza sativa
6.2.1.8 OCS
-
 Oryza sativa
6.2.1.8 Oxalyl-CoA synthetase
-
 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.8 7.5
-
-
 Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.8 ATP
-
 Oryza sativa

General Information

EC Number General Information Comment Organism
6.2.1.8 metabolism the enzyme is involved in oxalate catabolism Oryza sativa