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Literature summary extracted from

  • Wang, C.; Guo, Y.; Tian, Q.; Jia, Q.; Gao, Y.; Zhang, Q.; Zhou, C.; Xie, W.
    SerRS-tRNASec complex structures reveal mechanism of the first step in selenocysteine biosynthesis (2015), Nucleic Acids Res., 43, 10534-10545 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.11 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.11 mutant enzymes E447K and E156T/R157S, sitting drop vapor diffusion method, using 12% (w/v) PEG 3350, 0.2 M NaCl and 0.1 MTris-HCl (pH 7.5) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.11 C117S the mutant shows increased activity compared to the wild type enzyme Homo sapiens
6.1.1.11 C46S the mutant shows increased activity compared to the wild type enzyme Homo sapiens
6.1.1.11 E156T/R157S the mutant retains about 10% of wild type activity Homo sapiens
6.1.1.11 E447K the mutant shows increased activity compared to the wild type enzyme Homo sapiens
6.1.1.11 G136V the mutant retains about 15% of wild type activity Homo sapiens
6.1.1.11 P30G31Y the mutation of the P30G31 dipeptide to a single tyrosine almost eliminates serylation activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.11 GSSG about 16% residual activity at 1 mM Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.11 cytoplasm
-
Homo sapiens 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.11 115000 154000 dynamic light scattering Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer Homo sapiens
-
AMP + diphosphate + L-seryl-tRNASer
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.11 Homo sapiens P49591
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.11 Ni-NTA column chromatography, Q-HP column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer
-
Homo sapiens AMP + diphosphate + L-seryl-tRNASer
-
?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.11 SARS
-
Homo sapiens
6.1.1.11 SerRS
-
Homo sapiens
6.1.1.11 Seryl-tRNA synthetase
-
Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.11 ATP
-
Homo sapiens