EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.27 | expressed in Escherichia coli B834(DE3) cells | Methanocaldococcus jannaschii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.73 | structure of the SepCysE-SepCysS-tRNACys ternary complex, at a resolution of 2.6 A by molecular replacement. SepCysS recognizes the discriminator base U73 of tRNACys | Methanocaldococcus jannaschii |
6.1.1.27 | hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 7.0, 0.3M ammonium sulfate, 36% (w/v) 2-methyl-2,4-pentanediol | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.27 | ATP + O-phospho-L-serine + tRNACys | Methanocaldococcus jannaschii | - |
AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.73 | Methanocaldococcus jannaschii | Q59072 | - |
- |
2.5.1.73 | Methanocaldococcus jannaschii DSM 2661 | Q59072 | - |
- |
6.1.1.27 | Methanocaldococcus jannaschii | Q59054 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.27 | ATP + O-phospho-L-serine + tRNACys | - |
Methanocaldococcus jannaschii | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.27 | homotetramer | - |
Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.27 | O-phosphoseryl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
6.1.1.27 | SepRS | - |
Methanocaldococcus jannaschii |
6.1.1.27 | sepS | - |
Methanocaldococcus jannaschii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.27 | ATP | - |
Methanocaldococcus jannaschii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.73 | physiological function | the three domains of enhancer SepCysE each bind O-phosphoseryl-tRNA synthetase SepRS, Sep-tRNA:Cys-tRNA synthase SepCysS, and tRNACys, respectively, which mediates the dynamic architecture of the transsulfursome and enables a global long-range channeling of tRNACys between SepRS and SepCysS distant active sites | Methanocaldococcus jannaschii |