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Literature summary extracted from

  • Agarwal, V.; El Gamal, A.; Yamanaka, K.; Poth, D.; Kersten, R.; Schorn, M.; Allen, E.; Moore, B.
    Biosynthesis of polybrominated aromatic organic compounds by marine bacteria (2014), Nat. Chem. Biol., 10, 640-647 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.55 expression in Escherichia coli Pseudoalteromonas luteoviolacea
1.14.19.57 expression in Escherichia coli Pseudoalteromonas luteoviolacea

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.55 Pseudoalteromonas luteoviolacea
-
-
-
1.14.19.55 Pseudoalteromonas luteoviolacea 2ta16
-
-
-
1.14.19.57 Pseudoalteromonas luteoviolacea
-
-
-
1.14.19.57 Pseudoalteromonas luteoviolacea 2ta16
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.55 3-bromo-4-hydroxybenzoate + NADPH + bromide + O2 + H+
-
 Pseudoalteromonas luteoviolacea 2,4-dibromophenol + NADP+ + CO2 + 2 H2O
-
 ?
1.14.19.55 3-bromo-4-hydroxybenzoate + NADPH + bromide + O2 + H+
-
 Pseudoalteromonas luteoviolacea 2ta16 2,4-dibromophenol + NADP+ + CO2 + 2 H2O
-
 ?
1.14.19.55 4-hydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
-
 Pseudoalteromonas luteoviolacea 2,4-dibromophenol + 2 NADP+ + CO2 + 4 H2O
-
 ?
1.14.19.55 4-hydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
-
 Pseudoalteromonas luteoviolacea 2ta16 2,4-dibromophenol + 2 NADP+ + CO2 + 4 H2O
-
 ?
1.14.19.55 4-hydroxybenzoate + 2 NADPH + 2 iodide + 2 O2 + 2 H+
-
 Pseudoalteromonas luteoviolacea 2,4-diiodophenol + 2 NADP+ + CO2 + 4 H2O
-
 ?
1.14.19.55 4-hydroxybenzoate + 2 NADPH + 2 iodide + 2 O2 + 2 H+
-
 Pseudoalteromonas luteoviolacea 2ta16 2,4-diiodophenol + 2 NADP+ + CO2 + 4 H2O
-
 ?
1.14.19.55 4-hydroxybenzoate + NADPH + bromide + O2 + H+
-
 Pseudoalteromonas luteoviolacea 3-bromo-4-hydroxybenzoate + NADP+ + 2 H2O
-
 ?
1.14.19.55 4-hydroxybenzoate + NADPH + bromide + O2 + H+
-
 Pseudoalteromonas luteoviolacea 2ta16 3-bromo-4-hydroxybenzoate + NADP+ + 2 H2O
-
 ?
1.14.19.55 additional information no substrate: chloride Pseudoalteromonas luteoviolacea ?
-
 ?
1.14.19.55 additional information no substrate: chloride Pseudoalteromonas luteoviolacea 2ta16 ?
-
 ?
1.14.19.57 1H-pyrrole-2-carbonyl-[Bmp1 peptidyl-carrier protein] + 3 FADH2 + 3 bromide + 3 O2
-
 Pseudoalteromonas luteoviolacea 3,4,5-tribromo-1H-pyrrole-2-carbonyl-[Bmp1 peptidyl-carrier protein] + 3 FAD + 6 H2O
-
 ?
1.14.19.57 1H-pyrrole-2-carbonyl-[Bmp1 peptidyl-carrier protein] + FADH2 + bromide + O2
-
 Pseudoalteromonas luteoviolacea 5-bromo-1H-pyrrole-2-carbonyl-[Bmp1 peptidyl-carrier protein] + FAD + 2 H2O
-
 ?
1.14.19.57 4,5-dibromo-1H-pyrrole-2-carbonyl-[Bmp1 peptidyl-carrier protein] + FADH2 + bromide + O2
-
 Pseudoalteromonas luteoviolacea 3,4,5-tribromo-1H-pyrrole-2-carbonyl-[Bmp1 peptidyl-carrier protein] + FAD + 2 H2O
-
 ?
1.14.19.57 5-bromo-1H-pyrrole-2-carbonyl-[Bmp1 peptidyl-carrier protein] + FADH2 + bromide + O2
-
 Pseudoalteromonas luteoviolacea 4,5-dibromo-1H-pyrrole-2-carbonyl-[Bmp1 peptidyl-carrier protein] + FAD + 2 H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.55 FAD Bmp5 does not require the addition of a flavin reductase to regenerate FADH2 in situ Pseudoalteromonas luteoviolacea
1.14.19.55 NADPH absolutely required Pseudoalteromonas luteoviolacea
1.14.19.57 FAD
-
 Pseudoalteromonas luteoviolacea
1.14.19.57 NADPH
-
 Pseudoalteromonas luteoviolacea

General Information

EC Number General Information Comment Organism
1.14.19.55 physiological function deletion of the Bmp5 gene eliminates the synthesis of bromophenol-containing species Pseudoalteromonas luteoviolacea
1.14.19.57 physiological function deletion of gene Bmp2 abolishes the production of bromopyrroles. In the presence of Escherichia coli flavin reductase SsuE, NADPH and bromide, Bmp2 catalyzes the bromination of pyrrolyl-S-Bmp1(acyl carrier protein) to mono-, di- and tribromopyrrolyl-S-Bmp1(acyl carrier protein) Pseudoalteromonas luteoviolacea