Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wronska, N.; Brzostek, A.; Szewczyk, R.; Sobon, A.; Dziadek, J.; Lisowska, K.
    The role of fadD19 and echA19 in sterol side chain degradation by mycobacterium smegmatis (2016), Molecules, 21, 598-611 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.42 gene fadD19, DNA and amino acid sequence determination and analysis, chromosomal localization, expression analysis Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.42 additional information construction of complemented mutant carrying an intact fadD19 under control of its natural putative promoter (Mycobacterium smegmatis DELTAfadD19; attB::PfadD19fadD19). The complemented strain, but not the DELTAfadD19 mutant, is able to degrade beta-sitosterol with kinetics similar to that of wild-type Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.42 Mg2+ required Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.42 ATP + (25S)-3-oxocholest-4-en-26-oate + CoA Mycolicibacterium smegmatis
-
AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA
-
?
6.2.1.42 ATP + (25S)-3-oxocholest-4-en-26-oate + CoA Mycolicibacterium smegmatis mc2155
-
AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.42 Mycolicibacterium smegmatis I7FZL8
-
-
6.2.1.42 Mycolicibacterium smegmatis mc2155 I7FZL8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.42 ATP + (25S)-3-oxocholest-4-en-26-oate + CoA
-
Mycolicibacterium smegmatis AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA
-
?
6.2.1.42 ATP + (25S)-3-oxocholest-4-en-26-oate + CoA
-
Mycolicibacterium smegmatis mc2155 AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.42 fadD19
-
Mycolicibacterium smegmatis
6.2.1.42 steroid-CoA ligase
-
Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.42 ATP
-
Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
6.2.1.42 metabolism the enzyme plays an essential role in C-24 branched side chain sterol degradation, overview Mycolicibacterium smegmatis
6.2.1.42 physiological function the enzyme encoded by gene fadD19 plays a role in the degradation process of the side chain of cholesterol and beta-sitosterol, but is not essential for viability. FadD19 is required in the initial step of the biodegradation of C-24 branched sterol side chains in Mycobacterium smegmatis mc2155, like beta-sitosterol, but not those carrying a straight chain, like cholesterol. The enzyme has an essential role of FadD19 in the early step of beta-sitosterol degradation Mycolicibacterium smegmatis