Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Garba, L.; Ali, M.S.; Oslan, S.N.; Rahman, R.N.
    Heterologous expression of PA8FAD9 and functional characterization of a DELTA9-fatty acid desaturase from a cold-tolerant Pseudomonas sp. A8 (2016), Mol. Biotechnol., 58, 718-728 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.28 gene PA8FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain Transetta (DE3), ability of the enzyme to convert exogenous stearic acid to oleic acid incorporated into the recombinant Escherichia coli membrane phospholipids. GCMS analysis confirms significant changes in the pattern of palmitoleic and palmitic acid production in Escherichia coli cells expressing PA8FAD9 gene. At 20°C, the amount of palmitoleic increased from 10.5 to 21% suggesting that PA8FAD9 gene from Pseudomonas sp. A8 codes for an active DELTA9-fatty acid desaturase-like protein Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.28 membrane
-
 Pseudomonas sp. 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.28 1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Pseudomonas sp.
-
 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.28 1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Pseudomonas sp. A8(2016b)
-
 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.28 Pseudomonas sp. A0A165DNG6
-
-
1.14.19.28 Pseudomonas sp. A8(2016b) A0A165DNG6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.28 1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Pseudomonas sp. 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.28 1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Pseudomonas sp. A8(2016b) 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.28 ? x * 45000, about, sequence calculation Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.19.28 DELTA9-fatty acid desaturase
-
 Pseudomonas sp.
1.14.19.28 DELTA9-fatty acid desaturase-like protein
-
 Pseudomonas sp.
1.14.19.28 FAD9
-
 Pseudomonas sp.
1.14.19.28 PA8FAD9
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.28 Ferredoxin
-
 Pseudomonas sp.

General Information

EC Number General Information Comment Organism
1.14.19.28 physiological function the D9-fatty acid desaturase initiates the synthesis of polyunsaturated fatty acids by introducing the first double bond at the C9 position of saturated fatty acid chains. These substrates can further be elongated by other classes of desaturase enzymes Pseudomonas sp.