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Literature summary extracted from

  • Larsen, O.; Karlsen, O.A.
    Transcriptomic profiling of Methylococcus capsulatus (Bath) during growth with two different methane monooxygenases (2016), MicrobiologyOpen, 5, 254-267 .
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.25 cytoplasm
-
Methylococcus capsulatus 5737
-
1.14.18.3 membrane
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Methylococcus capsulatus 16020
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Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.25 additional information the soluble methane monooxygenase contains no copper Methylococcus capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.25 methane + NADH + H+ + O2 Methylococcus capsulatus
-
methanol + NAD+ + H2O
-
?
1.14.13.25 methane + NADH + H+ + O2 Methylococcus capsulatus Bath
-
methanol + NAD+ + H2O
-
?
1.14.13.25 methane + NADH + H+ + O2 Methylococcus capsulatus Bath.
-
methanol + NAD+ + H2O
-
?
1.14.18.3 methane + quinol + O2 Methylococcus capsulatus
-
methanol + quinone + H2O
-
?
1.14.18.3 methane + quinol + O2 Methylococcus capsulatus Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). Major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells. Quinones are predominantly used as the electron donors for methane oxidation by pMMO. During production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation methanol + quinone + H2O
-
?
1.14.18.3 methane + quinol + O2 Methylococcus capsulatus Bath Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). Major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells. Quinones are predominantly used as the electron donors for methane oxidation by pMMO. During production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation methanol + quinone + H2O
-
?
1.14.18.3 methane + quinol + O2 Methylococcus capsulatus Bath
-
methanol + quinone + H2O
-
?
1.14.18.3 methane + quinol + O2 Methylococcus capsulatus Bath. Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). Major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells. Quinones are predominantly used as the electron donors for methane oxidation by pMMO. During production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation methanol + quinone + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylococcus capsulatus
-
-
-
1.14.13.25 Methylococcus capsulatus P22869 and P18798 and P11987 and P18797 and P22868 and P22867 P22869 (MmoX), P18798 (MmoY), P11987 (MmoZ), P18797 (MmoB), P22868 (MmoC), P22867 (MmoD). The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD)
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1.14.13.25 Methylococcus capsulatus Bath
-
-
-
1.14.13.25 Methylococcus capsulatus Bath. P22869 and P18798 and P11987 and P18797 and P22868 and P22867 P22869 (MmoX), P18798 (MmoY), P11987 (MmoZ), P18797 (MmoB), P22868 (MmoC), P22867 (MmoD). The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD)
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1.14.18.3 Methylococcus capsulatus
-
-
-
1.14.18.3 Methylococcus capsulatus G1UBD1 AND Q607G3 alpha- and beta-subunits
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1.14.18.3 Methylococcus capsulatus Bath G1UBD1 AND Q607G3 alpha- and beta-subunits
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1.14.18.3 Methylococcus capsulatus Bath.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.25 additional information transcriptomic profiling of soluble MMO is used to investigate the gene expression pattern in Methylococcus capsulatus during methanotrophic growth in the presence and absence of copper, overview Methylococcus capsulatus
-
1.14.18.3 additional information transcriptomic profiling of particulate MMO is used to investigate the gene expression pattern in Methylococcus capsulatus during methanotrophic growth in the presence and absence of copper, overview Methylococcus capsulatus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NADH + H+ + O2
-
Methylococcus capsulatus methanol + NAD+ + H2O
-
?
1.14.13.25 methane + NADH + H+ + O2
-
Methylococcus capsulatus Bath methanol + NAD+ + H2O
-
?
1.14.13.25 methane + NADH + H+ + O2
-
Methylococcus capsulatus Bath. methanol + NAD+ + H2O
-
?
1.14.18.3 methane + quinol + O2
-
Methylococcus capsulatus methanol + quinone + H2O
-
?
1.14.18.3 methane + quinol + O2 Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). Major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells. Quinones are predominantly used as the electron donors for methane oxidation by pMMO. During production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation Methylococcus capsulatus methanol + quinone + H2O
-
?
1.14.18.3 methane + quinol + O2 Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). Major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells. Quinones are predominantly used as the electron donors for methane oxidation by pMMO. During production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation Methylococcus capsulatus Bath methanol + quinone + H2O
-
?
1.14.18.3 methane + quinol + O2
-
Methylococcus capsulatus Bath methanol + quinone + H2O
-
?
1.14.18.3 methane + quinol + O2 Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). Major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells. Quinones are predominantly used as the electron donors for methane oxidation by pMMO. During production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation Methylococcus capsulatus Bath. methanol + quinone + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 sMMO
-
Methylococcus capsulatus
1.14.13.25 soluble methane monooxygenase
-
Methylococcus capsulatus
1.14.18.3 membrane-embedded methane monooxygenase
-
Methylococcus capsulatus
1.14.18.3 pMMO
-
Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
Methylococcus capsulatus
1.14.18.3 quinol quinone is predominantly used by pMMO in order to sustain its activity Methylococcus capsulatus

General Information

EC Number General Information Comment Organism
1.14.13.25 metabolism the enzyme expresses the soluble enzyme form under copper limitation, and the membrane-bound particulate MMO at high copper-to-biomass ratio, analysis of the mechanism of the copper switch. Transcriptomic profiling of particulate MMO, EC 1.14.18.3, and soluble MMO, using Methylococcus capsulatus DNA microarrays. 137 ORFs are found to be differentially expressed between cells producing sMMO and pMMO, while only minor differences in gene expression are observed between the pMMO-producing cultures. Of these, 87 genes are upregulated during sMMO-producing cells, i.e. during copper-limited growth. Major changes takes place in the respiratory chain between pMMO-and sMMO-producing cells, and quinone are predominantly used as the electron donors for methane oxidation by pMMO. Proposed pathway of methane oxidation in Methylococcus capsulatus cells producing either sMMO or pMMO, overview Methylococcus capsulatus
1.14.13.25 metabolism the high number of up-regulated genes in cells producing soluble methane monooxygenase shows that Methylococcus capsulatus is highly adapted to copper-limited growth Methylococcus capsulatus
1.14.18.3 metabolism during production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation Methylococcus capsulatus
1.14.18.3 metabolism the enzyme expresses the soluble enzyme form under copper limitation, and the membrane-bound particulate MMO at high copper-to-biomass ratio, analysis of the mechanism of the copper switch. Transcriptomic profiling of particulate MMO and soluble MMO, EC 1.14.13.25, using Methylococcus capsulatus DNA microarrays. 137 ORFs are found to be differentially expressed between cells producing sMMO and pMMO, while only minor differences in gene expression are observed between the pMMO-producing cultures. Of these, 87 genes are upregulated during sMMO-producing cells, i.e. during copper-limited growth. Major changes takes place in the respiratory chain between pMMO-and sMMO-producing cells, and quinone are predominantly used as the electron donors for methane oxidation by pMMO. Proposed pathway of methane oxidation in Methylococcus capsulatus cells producing either sMMO or pMMO, overview Methylococcus capsulatus