Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Connelly, K.R.; Stevenson, C.; Kneuper, H.; Sargent, F.
    Biosynthesis of selenate reductase in Salmonella enterica critical roles for the signal peptide and DmsD (2016), Microbiology, 162, 2136-2146 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.97.1.9 gene ynfE, encoded by the ynfEFGH-dmsD operon, the YnfE protein is synthesized with an N-terminal twin-arginine signal peptide and biosynthesis of the enzyme is coordinated by a signal peptide binding chaperone called DmsD Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
1.97.1.9 A28Q site-directed mutagenesis, the mutant is devoid of selenate reductase activity and can be rescued by providing chaperon protein DmsD in excess Salmonella enterica subsp. enterica serovar Typhimurium
1.97.1.9 F35Q site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium
1.97.1.9 L24Q site-directed mutagenesis, the mutant is devoid of selenate reductase activity Salmonella enterica subsp. enterica serovar Typhimurium
1.97.1.9 L33Q site-directed mutagenesis, the mutant is still able to correctly assemble selenate reductase Salmonella enterica subsp. enterica serovar Typhimurium
1.97.1.9 V31Q site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.97.1.9 membrane membrane-bound enzyme Salmonella enterica subsp. enterica serovar Typhimurium 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.97.1.9 selenate + reduced acceptor Salmonella enterica subsp. enterica serovar Typhimurium
-
 selenite + H2O + acceptor
-
 ?
1.97.1.9 selenate + reduced acceptor Salmonella enterica subsp. enterica serovar Typhimurium LT2a
-
 selenite + H2O + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.97.1.9 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
1.97.1.9 Salmonella enterica subsp. enterica serovar Typhimurium LT2a
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.97.1.9 selenate + reduced acceptor
-
 Salmonella enterica subsp. enterica serovar Typhimurium selenite + H2O + acceptor
-
 ?
1.97.1.9 selenate + reduced acceptor
-
 Salmonella enterica subsp. enterica serovar Typhimurium LT2a selenite + H2O + acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.97.1.9 heterotrimer the active enzyme is predicted to comprise at least three subunits where YnfE is a molybdenum-containing catalytic subunit Salmonella enterica subsp. enterica serovar Typhimurium
1.97.1.9 More YnfE signal peptide residues L24 and A28 are important for assembly of an active selenate reductase Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
1.97.1.9 YnfE
-
 Salmonella enterica subsp. enterica serovar Typhimurium

General Information

EC Number General Information Comment Organism
1.97.1.9 additional information the signal peptide binding chaperone that interacts with the selenate reductase (YnfE) signal peptide is called DmsD and it is encoded within the ynfEFGH-dmsD operon. YnfE signal peptide residues L24 and A28 are important for assembly of an active selenate reductase, and DmsD V16 residue is important for signal peptide recognition and selenate reductase assembly, random mutational analysis. DmsD is a member of the TorD/DmsD/NarJ family of peptide binding proteins. Synthetic peptides containing the YnfE binding epitope can be chemically crosslinked to DmsD and signal peptide binding prevents DmsD dimerization in vitro, interaktion analysis, overview Salmonella enterica subsp. enterica serovar Typhimurium