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Literature summary extracted from

  • Woelke, M.R.; Paulucci, N.S.; Selva, A.; Garban, H.; de Lema, M.G.
    DELTA9 desaturase from Trypanosoma cruzi key enzyme in the parasite metabolism. Cloning and overexpression (2017), Microbiol. Res., 194, 29-37 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.28 DNA and amino acid sequence determination and analysis, functional overexpression of His6-tagged enzyme in Escherichia coli strain BL21 codon Plus, subcloning in Escherichia coli strain XL 10 GOLD, TcDELTA9DES-expressing Escherichia coli cells exhibit high desaturase activity associated with increased levels of monounsaturated fatty acids. The fatty acid composition of Escherichia coli shows that the recombinant desaturase can use 16:0 as substrate Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.19.28 ketoconazole
-
 Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.28 membrane membrane-bound Trypanosoma cruzi 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.28 1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Trypanosoma cruzi
-
 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.28 1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Trypanosoma cruzi Tulahuen
-
 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.28 Trypanosoma cruzi G8FL60
-
-
1.14.19.28 Trypanosoma cruzi Tulahuen G8FL60
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.28 native enzyme from epimastigotes partially by ultracentrifugation, recombinant His6-tagged enzyme from Escherichia coli strain BL21 codon Plus cell-free extract by nickel affinity chromatography Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.28 epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.28 1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Trypanosoma cruzi 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.28 1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Trypanosoma cruzi Tulahuen 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.28 ? x * 50000, about, recombinant enzyme, SDS-PAGE Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
1.14.19.28 9des
-
 Trypanosoma cruzi
1.14.19.28 DELTA9 desaturase
-
 Trypanosoma cruzi
1.14.19.28 TcDELTA9DES
-
 Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.28 37
-
 in vivo assay at Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.28 Ferredoxin
-
 Trypanosoma cruzi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.19.28 Trypanosoma cruzi about, isoelectric focusing
-
 9

General Information

EC Number General Information Comment Organism
1.14.19.28 metabolism desaturases, key enzymes in the metabolism of fatty acids, regulate the physical and biochemical properties of membranes. They adjust the composition of saturated and unsaturated fatty acids in response to changes in the environmental. This DELTA9-desaturation reaction normally requires molecular oxygen and reducing equivalents derived from NAD(P)H through an electron system consisting of either NADPH, cytochrome b5 and cytochrome b5-reductase in animals and fungi or ferredoxin-NADP+ oxidoreductase and ferredoxin in bacteria and plants Trypanosoma cruzi
1.14.19.28 physiological function the enzyme plays a critical role in the metabolism of the parasite Trypanosoma cruzi