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Literature summary extracted from

  • Kumar, V.; Yadav, S.; Soumya, N.; Kumar, R.; Babu, N.K.; Singh, S.
    Biochemical and inhibition studies of glutamine synthetase from Leishmania donovani (2017), Microb. Pathog., 107, 164-174 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 gene LdGS, the enzyme exists as a single copy gene in parasite genome, sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 L-methionine sulfone
-
 Leishmania donovani
6.3.1.2 L-methionine sulfoximine
-
 Leishmania donovani
6.3.1.2 Phosphinothricin
-
 Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 26.3
-
 L-glutamate recombinant enzyme, pH 7.8, 37°C Leishmania donovani

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.2 cytoplasm
-
 Leishmania donovani 5737
-
6.3.1.2 additional information presence of mitochondrial targeting sequence and the cleavage localization signal at the N-terminal region in LdGS protein Leishmania donovani
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Ca2+ can partially substitute for Mg2+ Leishmania donovani
6.3.1.2 Mg2+ required Leishmania donovani
6.3.1.2 Mn2+ can partially substitute for Mg2+ Leishmania donovani
6.3.1.2 Ni2+ can partially substitute for Mg2+ Leishmania donovani
6.3.1.2 Zn2+ can partially substitute for Mg2+ Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3 Leishmania donovani
-
 ADP + phosphate + L-glutamine
-
 r
6.3.1.2 ATP + L-glutamate + NH3 Leishmania donovani BPK282A1
-
 ADP + phosphate + L-glutamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Leishmania donovani E9B8P8 MHOM/80/IN/Dd8
-
6.3.1.2 Leishmania donovani BPK282A1 E9B8P8 MHOM/80/IN/Dd8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Leishmania donovani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.2 amastigote
-
 Leishmania donovani
-
6.3.1.2 promastigote
-
 Leishmania donovani
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 0.322
-
 enzyme from promastigotes, pH 7.8, 37°C Leishmania donovani
6.3.1.2 0.7325
-
 recombinant His-tagged enzyme, pH 7.8, 37°C Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3
-
 Leishmania donovani ADP + phosphate + L-glutamine
-
 r
6.3.1.2 ATP + L-glutamate + NH3
-
 Leishmania donovani BPK282A1 ADP + phosphate + L-glutamine
-
 r

Subunits

EC Number Subunits Comment Organism
6.3.1.2 ? x * 43500, recombinant His-tagged enzyme, SDS-PAGE Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
6.3.1.2 LDBPK_060370
-
 Leishmania donovani
6.3.1.2 LdGS
-
 Leishmania donovani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 37
-
 assay at Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 8
-
-
 Leishmania donovani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.1.2 6 11 activity range Leishmania donovani

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.2 ATP
-
 Leishmania donovani