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Literature summary extracted from

  • Yeh, C.H.; Kuo, Y.S.; Chang, C.M.; Liu, W.H.; Sheu, M.L.; Meng, M.
    Deletion of the gene encoding the reductase component of 3-ketosteroid 9alpha-hydroxylase in Rhodococcus equi USA-18 disrupts sterol catabolism, leading to the accumulation of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid and 1,4-androstadiene-3,17-dione (2014), Microb. Cell Fact., 13, 130 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.30 gene A5N73_13410, sequence comparisons Rhodococcus equi

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.30 additional information deletion of the gene encoding the reductase component KshB of 3-ketosteroid 9alpha-hydroxylase in Rhodococcus equi strain USA-18 by a PCR-targeted gene disruption method results in a mutant strain that accumulates up to 0.58 mg/ml 1,4-androstadiene-3,17-dione in the culture medium when 0.2% cholesterol is used as the carbon source. The mutant also accumulates 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid (DELTA1,4-BNC). 1,4-Androstadiene-3,17-dione in strain USA-18DELTAB8 preferentially imposes a feedback inhibition on the enzymes involved in the side chain oxidation of DELTA1,4-BNC, but not DELTA4-BNC. Mutant phenotypes, overview Rhodococcus equi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.30 3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Rhodococcus equi
-
 9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Rhodococcus equi 103S
-
 9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Rhodococcus equi
-
 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Rhodococcus equi 103S
-
 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.30 Rhodococcus equi A0A060CNE1 i.e. Rhodococcus hoagii
-
1.14.15.30 Rhodococcus equi 103S A0A060CNE1 i.e. Rhodococcus hoagii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.30 3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus equi 9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus equi 103S 9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus equi 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus equi 103S 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.30 3-ketosteroid 9alpha-hydroxylase
-
 Rhodococcus equi
1.14.15.30 A5N73_13410
-
 Rhodococcus equi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.30 37
-
 in vivo assay at Rhodococcus equi

General Information

EC Number General Information Comment Organism
1.14.15.30 malfunction deletion of the gene encoding the reductase component KshB of 3-ketosteroid 9alpha-hydroxylase in Rhodococcus equi strain USA-18 disrupts sterol catabolism, leading to the accumulation of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid and 1,4-androstadiene-3,17-dione Rhodococcus equi