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Literature summary extracted from
- Substrate scope of a dehydrogenase from Sphingomonas species A1 and its potential application in the synthesis of rare sugars and sugar derivatives (2018), Microb. Biotechnol., 11, 747-758 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.1 | expressed in Escherichia coli BL21(DE3) cells | Sphingomonas sp. A1 |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.1 | 6 | - |
D-talonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 |  |
| 1.1.1.1 | 12 | - |
D-gluconate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 13 | - |
2-dehydro-3-deoxy-D-gluconate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 14 | - |
D-mannonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 16 | - |
D-ribonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 16 | - |
D-allonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 24 | - |
D-xylonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 24 | - |
D-altronate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 220 | - |
D-arabitol | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 350 | - |
D-sorbitol | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.1 | 2-dehydro-3-deoxy-D-gluconate + NADH + H+ | Sphingomonas sp. A1 | - |
4-deoxy-L-erythro-5-hexoseulose + NAD+ | - |
r | |
| 1.1.1.1 | 4-deoxy-L-erythro-5-hexoseulose + NAD+ | Sphingomonas sp. A1 | preferred reaction | 2-dehydro-3-deoxy-D-gluconate + NADH + H+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.1 | Sphingomonas sp. A1 | A0A075B5H4 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.1 | 2-dehydro-3-deoxy-D-gluconate + NADH + H+ | - |
Sphingomonas sp. A1 | 4-deoxy-L-erythro-5-hexoseulose + NAD+ | - |
r | |
| 1.1.1.1 | 4-deoxy-L-erythro-5-hexoseulose + NAD+ | 100% activity | Sphingomonas sp. A1 | 2-dehydro-3-deoxy-D-gluconate + NADH + H+ | - |
r | |
| 1.1.1.1 | 4-deoxy-L-erythro-5-hexoseulose + NAD+ | preferred reaction | Sphingomonas sp. A1 | 2-dehydro-3-deoxy-D-gluconate + NADH + H+ | - |
r | |
| 1.1.1.1 | D-allonate + NADH + H+ | 10% activity compared to 2-dehydro-3-deoxy-D-gluconate | Sphingomonas sp. A1 | ? | - |
? | |
| 1.1.1.1 | D-altronate + NADH + H+ | 8% activity compared to 2-dehydro-3-deoxy-D-gluconate | Sphingomonas sp. A1 | ? | - |
? | |
| 1.1.1.1 | D-arabitol + NADH + H+ | - |
Sphingomonas sp. A1 | D-lyxose + D-arabinose + NAD+ | - |
? | |
| 1.1.1.1 | D-erythrose + NAD+ | - |
Sphingomonas sp. A1 | ? | - |
? | |
| 1.1.1.1 | D-gluconate + NADH + H+ | 79% activity compared to 2-dehydro-3-deoxy-D-gluconate | Sphingomonas sp. A1 | ? | - |
? | |
| 1.1.1.1 | D-glyceraldehyde + NAD+ | - |
Sphingomonas sp. A1 | ? | - |
? | |
| 1.1.1.1 | D-mannonate + NADH + H+ | 37% activity compared to 2-dehydro-3-deoxy-D-gluconate | Sphingomonas sp. A1 | ? | - |
? | |
| 1.1.1.1 | D-ribonate + NADH + H+ | 0.3% activity compared to 2-dehydro-3-deoxy-D-gluconate | Sphingomonas sp. A1 | ? | - |
? | |
| 1.1.1.1 | D-sorbitol + NADH + H+ | 0.01% activity compared to 2-dehydro-3-deoxy-D-gluconate | Sphingomonas sp. A1 | L-gulose + NAD+ | - |
? | |
| 1.1.1.1 | D-talonate + NADH + H+ | 3% activity compared to 2-dehydro-3-deoxy-D-gluconate | Sphingomonas sp. A1 | ? | - |
? | |
| 1.1.1.1 | D-threose + NAD+ | - |
Sphingomonas sp. A1 | ? | - |
? | |
| 1.1.1.1 | D-xylonate + NADH + H+ | 1% activity compared to 2-dehydro-3-deoxy-D-gluconate | Sphingomonas sp. A1 | ? | - |
? | |
| 1.1.1.1 | L-glyceraldehyde + NAD+ | - |
Sphingomonas sp. A1 | ? | - |
? | |
| 1.1.1.1 | additional information | almost no activity with D-arabinonate, D-lyxonate, D-galactonate, glycerol, meso-erythritol, D-ribitol, D-arabitol, D-xylitol, and D-mannitol. No activity with propanal, butanal, hexanal, and 4-oxobutanoic acid | Sphingomonas sp. A1 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.1 | ADH | - |
Sphingomonas sp. A1 |
| 1.1.1.1 | NAD+-dependent SDR | - |
Sphingomonas sp. A1 |
| 1.1.1.1 | short-chain dehydrogenase/reductase | - |
Sphingomonas sp. A1 |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.1 | 25 | - |
the half-life of the enzyme is 20 h at 25°C in ammonium bicarbonate buffer pH 7.9 with 1 mM NAD+ | Sphingomonas sp. A1 |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.1 | 0.0019 | - |
D-arabitol | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.0052 | - |
D-sorbitol | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.0052 | - |
D-ribonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.021 | - |
D-talonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.032 | - |
D-xylonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.18 | - |
D-allonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.19 | - |
D-altronate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.53 | - |
D-mannonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.98 | - |
D-gluconate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 1.4 | - |
2-dehydro-3-deoxy-D-gluconate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.1 | NAD+ | dependent on | Sphingomonas sp. A1 | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.1 | metabolism | the enzyme is involved in alginate metabolism | Sphingomonas sp. A1 |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.1 | 0.000015 | - |
D-sorbitol | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.000087 | - |
D-arabitol | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.00033 | - |
D-ribonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.0014 | - |
D-xylonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.0034 | - |
D-talonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.008 | - |
D-altronate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.011 | - |
D-allonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.038 | - |
D-mannonate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.082 | - |
D-gluconate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
| 1.1.1.1 | 0.1 | - |
2-dehydro-3-deoxy-D-gluconate | at pH 7.9 and 25°C | Sphingomonas sp. A1 | |
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