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Literature summary extracted from

  • Rodionova, I.A.; Schuster, B.M.; Guinn, K.M.; Sorci, L.; Scott, D.A.; Li, X.; Kheterpal, I.; Shoen, C.; Cynamon, M.; Locher, C.; Rubin, E.J.; Osterman, A.L.
    Metabolic and bactericidal effects of targeted suppression of NadD and NadE enzymes in mycobacteria (2014), mBio, 5, e00747-13 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.3.1.5 drug development NadE is a antimycobacterial drug target Mycolicibacterium smegmatis
6.3.1.5 drug development NadE is a antimycobacterial drug target Mycobacterium tuberculosis
6.3.1.5 pharmacology NadE is a antimycobacterial drug target Mycolicibacterium smegmatis
6.3.1.5 pharmacology NadE is a antimycobacterial drug target Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
6.3.1.5 additional information knockout of gene nadE Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.5 Mg2+ required Mycolicibacterium smegmatis
6.3.1.5 Mg2+ required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + NH3 Mycolicibacterium smegmatis
-
 AMP + diphosphate + NAD+
-
 ?
6.3.1.5 ATP + deamido-NAD+ + NH3 Mycobacterium tuberculosis
-
 AMP + diphosphate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.5 Mycobacterium tuberculosis A0A0U0RRS4
-
-
6.3.1.5 Mycolicibacterium smegmatis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + NH3
-
 Mycolicibacterium smegmatis AMP + diphosphate + NAD+
-
 ?
6.3.1.5 ATP + deamido-NAD+ + NH3
-
 Mycobacterium tuberculosis AMP + diphosphate + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.5 NAD synthetase
-
 Mycolicibacterium smegmatis
6.3.1.5 NAD synthetase
-
 Mycobacterium tuberculosis
6.3.1.5 NadE
-
 Mycolicibacterium smegmatis
6.3.1.5 NadE
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.5 ATP
-
 Mycolicibacterium smegmatis
6.3.1.5 ATP
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
6.3.1.5 malfunction induced degradation of NadE enzyme shows a strong bactericidal effect which coincided with anticipated changes in relative levels of NaAD intermediate (substrate of NadE) and ultimate depletion of the NAD(H) pool. The disruption of NAD production in the cell via genetic suppression of the essential enzymes (NadD and NadE) involved in the last two steps of NAD biogenesis leads to cell death, even under dormancy conditions. Proteolytic degradation of the enzyme leads to depletion of the NAD cofactor pool, which suppresses metabolic flux through numerous NAD(P)-dependent pathways of central metabolism of carbon and energy production, phenotype, overview Mycolicibacterium smegmatis
6.3.1.5 malfunction the disruption of NAD production in the cell via genetic suppression of the essential enzymes (NadD and NadE) involved in the last two steps of NAD biogenesis leads to cell death, even under dormancy conditions. Proteolytic degradation of either target enzyme leads to depletion of the NAD cofactor pool, which suppresses metabolic flux through numerous NAD(P)-dependent pathways of central metabolism of carbon and energy production Mycobacterium tuberculosis
6.3.1.5 metabolism NaMN adenylyltransferase (NadD) and NAD synthetase (NadE) are the key enzymes of NAD biosynthesis Mycolicibacterium smegmatis
6.3.1.5 metabolism NaMN adenylyltransferase (NadD) and NAD synthetase (NadE) are the key enzymes of NAD biosynthesis Mycobacterium tuberculosis