Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Guevara, G.; Heras, L.F.L.; Perera, J.; Llorens, J.M.N.
    Functional characterization of 3-ketosteroid 9alpha-hydroxylases in Rhodococcus ruber strain chol-4 (2017), J. Steroid Biochem. Mol. Biol., 172, 176-187 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.30 gene kshA, DNA and amino acid sequence determination and analysis, sequence comparisons Rhodococcus ruber

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.30 additional information construction of chol-4 enzyme DELTAkshA1,2,3 deletion mutants, genetic complementation of the DELTAkshA1,2,3 strain with KshA1 perfectly restores growth in CHO or cholic acid but not in androsta-4-ene-3,17-dione, androsta-1,4-diene-3,17-dione or DHEA, whereas the DELTAkshA2,3 does it to certain point. Mutant DELTAKshA3 can only grow using cholesterol as carbon source. KshA2 is only able to act on substrates with a short side chain Rhodococcus ruber

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.30 4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Rhodococcus ruber
-
 9alpha-hydroxy-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Rhodococcus ruber Chol-4
-
 9alpha-hydroxy-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Rhodococcus ruber
-
 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Rhodococcus ruber Chol-4
-
 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.30 Rhodococcus ruber
-
-
-
1.14.15.30 Rhodococcus ruber I0B6I9 KshA
-
1.14.15.30 Rhodococcus ruber Chol-4
-
-
-
1.14.15.30 Rhodococcus ruber Chol-4 I0B6I9 KshA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.30 1,4-androstadiene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber 9alpha-hydroxy-1,4-androstadiene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 1,4-androstadiene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber Chol-4 9alpha-hydroxy-1,4-androstadiene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus ruber 9alpha-hydroxy-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus ruber Chol-4 9alpha-hydroxy-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber 9alpha-hydroxy-4-androsten-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber Chol-4 9alpha-hydroxy-4-androsten-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus ruber 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus ruber Chol-4 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 cholestenone + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber ?
-
 ?
1.14.15.30 cholestenone + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber Chol-4 ?
-
 ?
1.14.15.30 cholic acid + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber ?
-
 ?
1.14.15.30 cholic acid + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber Chol-4 ?
-
 ?
1.14.15.30 dehydroepiandrosterone + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber ?
-
 ?
1.14.15.30 dehydroepiandrosterone + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber Chol-4 ?
-
 ?
1.14.15.30 dehydroepiandrosterone + NADH + H+ + O2
-
 Rhodococcus ruber 3beta,9alpha-dihydroxy-5-androstene-17-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 dehydroepiandrosterone + NADH + H+ + O2
-
 Rhodococcus ruber Chol-4 3beta,9alpha-dihydroxy-5-androstene-17-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 additional information isozyme KshA2 is only able to act on substrates with a short side chain in Rhodococcus ruber Rhodococcus ruber ?
-
 ?
1.14.15.30 additional information isozyme KshA2 is only able to act on substrates with a short side chain in Rhodococcus ruber Rhodococcus ruber Chol-4 ?
-
 ?
1.14.15.30 phytosterol + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber ?
-
 ?
1.14.15.30 pregnenolone + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber ?
-
 ?
1.14.15.30 sodium acetate + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber ?
-
 ?
1.14.15.30 testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Rhodococcus ruber ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.30 More enzyme domain structure, molecular modelling of KshA, structure comparisons of KshAB enzyme, detailed overview Rhodococcus ruber

Synonyms

EC Number Synonyms Comment Organism
1.14.15.30 3-ketosteroid 9alpha-hydroxylase
-
 Rhodococcus ruber
1.14.15.30 3-ketosteroid-9alpha-hydroxylase
-
 Rhodococcus ruber
1.14.15.30 androsta-1,4-diene-3,17-dione, NADH:oxygen oxidoreductase (9alpha-hydroxylating)
-
 Rhodococcus ruber
1.14.15.30 KshA
-
 Rhodococcus ruber
1.14.15.30 KshA1 isoform Rhodococcus ruber
1.14.15.30 KshA2 isoform Rhodococcus ruber
1.14.15.30 KshA3 isoform Rhodococcus ruber
1.14.15.30 KshAB
-
 Rhodococcus ruber
1.14.15.30 KshB
-
 Rhodococcus ruber

General Information

EC Number General Information Comment Organism
1.14.15.30 malfunction Rhodococcus ruber strains devoid of any KshAB activity (DELTAkshB or DELTAkshA1,2,3 strains) are able to grow in cholesterol although 10times slower than the wild-type strain. This growth is due to the degradation of the side chain, a process that has been described to occur at different steps of the pathway being independent of the polycyclic ring opening. In the strain the side chain degradation process does not depend on any KshAB activity Rhodococcus ruber
1.14.15.30 metabolism isoform KshA1 is a more versatile enzyme related to the cholic acid catabolism, although it also collaborates with KshA2 or KshA3 activities in the catabolism of steroids Rhodococcus ruber
1.14.15.30 additional information molecular modelling of KshA, structure comparisons of KshAB enzyme, detailed overview Rhodococcus ruber
1.14.15.30 physiological function 3-ketosteroid-9alpha-hydroxylase is a key enzyme in the general scheme of the bacterial steroid catabolism in combination with a 3-ketosteroid-DELTA1-dehydrogenase activity (KstD), being both responsible of the steroid nucleus (rings A/B) breakage. KshAB initiates the opening of the steroid ring by the 9alpha-hydroxylation of the C9 carbon of 4-ene-3-oxosteroids (e.g. AD) or 1,4-diene-3-oxosteroids (e.g. ADD), transforming them into 9alpha-hydroxy-4-androsten-3,17-dione or 9alpha-hydroxy-1,4-androstadiene-3,17-dione, respectively Rhodococcus ruber