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Literature summary extracted from

  • Gao, H.; Zhao, C.; Xiang, X.; Li, Y.; Zhao, Y.; Li, Z.; Pan, D.; Dai, Y.; Hara, H.; Cooper, D.K.; Cai, Z.; Mou, L.
    Production of alpha1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and handmade cloning (2017), J. Reprod. Dev., 63, 17-26 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.87 medicine development of alpha-1,3-galactosyltransferase (GGTA1) and cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH) double-knockout piglets as donor animals for xenotransplantation. Compared to cells from GGTA1-knockout pigs, human antibody binding and antibody-mediated complement-dependent cytotoxicity are significantly reduced in cells from GGTA1/CMAH double-knockout pigs Sus scrofa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.2 generation of bi-allelic GGTA1 and CMAH double-knockout cells Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
1.14.18.2 additional information production of alpha1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and cloning in porcine fetal fibroblast cells. Generation of bi-allelic GGTA1 and CMAH double-knockout cells, GGTA1/CMAH double-knockout (DKO) cells are cloned to produce piglets. Compared to cells from GGTA1-knockout pigs, human antibody binding and antibody-mediated complement-dependent cytotoxicity are significantly reduced in cells from GGTA1/CMAH DKO pigs, demonstrating that the pigs might exhibit reduced humoral rejection in xenotransplantation Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.2 cell surface
-
 Sus scrofa 9986
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.2 CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+ Sus scrofa
-
 CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.2 Sus scrofa
-
 Chinese domestic miniature Wuzhishan pigs
-
2.4.1.87 Sus scrofa P50127
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.2 CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Sus scrofa CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.2 CMAH
-
 Sus scrofa
1.14.18.2 cytidine monophosphate-N-acetylneuraminic acid hydroxylase
-
 Sus scrofa
2.4.1.87 GGTA1
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.2 cytochrome b5
-
 Sus scrofa

General Information

EC Number General Information Comment Organism
1.14.18.2 evolution detailed phylogenetic analysis, overview Sus scrofa
1.14.18.2 physiological function enzyme CMP-N-acetylneuraminic acid hydroxylase (CMAH) is responsible for the synthesis of N-glycolylneuraminic acid (Neu5Gc), a sialic acid present on the cell surface proteins of most deuterostomes. CMAH is the only enzyme capable of synthetizing Neu5Gc Sus scrofa