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Literature summary extracted from

  • Wang, X.; Cai, P.; Chen, K.; Ouyang, P.
    Efficient production of 5-aminovalerate from L-lysine by engineered Escherichia coli whole-cell biocatalysts (2016), J. Mol. Catal. B, 134, 115-121 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.13.12.2 synthesis installation of an Escherichia coli whole-cell biocatalytic system, environmentally friendly, for industrial production of 5-aminovalerate using recombinant enzymes 5-aminovaleramide amidohydrolase and L-lysine 2-monooxygenase, overview Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.2 gene davB, recombinant expression of codon-optimized enzyme DavB in Escherichia coli strains BL21(DE3) and BL-22A-RB-YB, coexpression with gene davA, encoding 5-aminovaleramide amidohydrolase Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.2 additional information generation of an optimized production system for 5-aminovalerate from L-lysine in Escherichia coli by overexpressing genes davA and davB, encoding 5-aminovaleramide amidohydrolase and L-lysine 2-monooxygenase, the effects of induction conditions, reaction temperature, metal ion additives, and cell permeability on the whole-cell biocatalyst system are evaluated to improve biocatalytic efficiency, overview. Presence of Mn2+ and Ca2+ enhances the activity of whole-cell BL-22A-RB-YB system. Increased permeabilization of Escherichia coli BL-22A-RB-YB cells following surfactant treatment with TritonX-100 results in improved L-lysine consumption and 5-aminovalerate synthesis rate Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.2 L-lysine + O2 Pseudomonas putida
-
 5-aminopentanamide + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.2 Pseudomonas putida Q88QV1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.2 L-lysine + O2
-
 Pseudomonas putida 5-aminopentanamide + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.2 davB
-
 Pseudomonas putida
1.13.12.2 L-lysine 2-monooxygenase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.2 37
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.2 7.5
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.2 FAD enzyme-bound, dependent on Pseudomonas putida