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Literature summary extracted from

  • Koseki, T.; Handa, H.; Watanabe, Y.; Ohtsuka, M.; Shiono, Y.
    An unusual feruloyl esterase from Aspergillus oryzae two tryptophan residues play a crucial role for the activity (2016), J. Mol. Catal. B, 133, S560-S568 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Pichia pastoris Aspergillus oryzae

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 W142F contrary to wild type, mutant is active against methyl esters of ferulic acid, p-coumaric acid, caffeic acid, and sinapic acid and ethyl ester of ferulic acid and exhibits enhanced production of ferulic acid from wheat arabinoxylan Aspergillus oryzae
3.1.1.73 W144Y contrary to wild type, mutant is active against methyl esters of ferulic acid, p-coumaric acid, caffeic acid, and sinapic acid and ethyl ester of ferulic acid and exhibits enhanced production of ferulic acid from wheat arabinoxylan Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.037
-
 methyl ferulate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.044
-
 ethyl ferulate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.05
-
 methyl p-coumarate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.057
-
 methyl caffeate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.067
-
 methyl ferulate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.086
-
 ethyl ferulate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.1
-
 methyl sinapinate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.11
-
 methyl p-coumarate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.12
-
 methyl caffeate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.15
-
 methyl sinapinate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.44
-
 alpha-naphthyl propionate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.45
-
 alpha-naphthyl propionate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.58
-
 alpha-naphthyl propionate wild-type, pH 7.0, 37°C Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular recombinant protein Aspergillus oryzae
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus oryzae Q2U7D3
-
-
3.1.1.73 Aspergillus oryzae ATCC 42149 Q2U7D3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 alpha-naphthyl propionate + H2O
-
 Aspergillus oryzae alpha-naphthol + propionate
-
 ?
3.1.1.73 alpha-naphthyl propionate + H2O
-
 Aspergillus oryzae ATCC 42149 alpha-naphthol + propionate
-
 ?
3.1.1.73 arabinoxylan + H2O
-
 Aspergillus oryzae ferulic acid + ?
-
 ?
3.1.1.73 arabinoxylan + H2O
-
 Aspergillus oryzae ATCC 42149 ferulic acid + ?
-
 ?
3.1.1.73 ethyl ferulate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae ethanol + ferulate
-
 ?
3.1.1.73 ethyl ferulate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae ATCC 42149 ethanol + ferulate
-
 ?
3.1.1.73 methyl caffeate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae methanol + caffeate
-
 ?
3.1.1.73 methyl ferulate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae methanol + ferulate
-
 ?
3.1.1.73 methyl ferulate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae ATCC 42149 methanol + ferulate
-
 ?
3.1.1.73 methyl p-coumarate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae methanol + p-coumarate
-
 ?
3.1.1.73 methyl sinapinate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae methanol + sinapinate
-
 ?
3.1.1.73 additional information wild-type rAoFaeD fails to cleave the methyl esters of ferulic acid, p-coumaric acid, caffeic acid, and sinapic acid and ethyl ester of ferulic acid Aspergillus oryzae ?
-
 ?
3.1.1.73 additional information wild-type rAoFaeD fails to cleave the methyl esters of ferulic acid, p-coumaric acid, caffeic acid, and sinapic acid and ethyl ester of ferulic acid Aspergillus oryzae ATCC 42149 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 30000, SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 AO090701000884
-
 Aspergillus oryzae
3.1.1.73 FaeD
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 45
-
-
 Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 0.18
-
 methyl caffeate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.23
-
 methyl ferulate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.31
-
 methyl caffeate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.35
-
 methyl p-coumarate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.51
-
 methyl ferulate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.57
-
 methyl p-coumarate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 0.68
-
 methyl sinapinate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 1.78
-
 methyl sinapinate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 3.79
-
 alpha-naphthyl propionate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 5.74
-
 alpha-naphthyl propionate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 5.8
-
 alpha-naphthyl propionate wild-type, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 71.6
-
 ethyl ferulate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 92.1
-
 ethyl ferulate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 7
-
-
 Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 7 10
-
 Aspergillus oryzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 2.6
-
 methyl caffeate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 3.2
-
 methyl caffeate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 5.5
-
 methyl p-coumarate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 6.2
-
 methyl ferulate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 6.7
-
 methyl sinapinate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 6.9
-
 methyl p-coumarate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 7.7
-
 methyl ferulate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 8.6
-
 alpha-naphthyl propionate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 10
-
 alpha-naphthyl propionate wild-type, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 11.9
-
 methyl sinapinate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 12.7
-
 alpha-naphthyl propionate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 1071
-
 ethyl ferulate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
3.1.1.73 1627
-
 ethyl ferulate mutant W142F, pH 7.0, 37°C Aspergillus oryzae