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Literature summary extracted from
- Extraction, partial purification and characterization of polyphenol oxidase from Solanum lycocarpum fruits (2014), J. Mol. Catal. B, 102, 211-217 .
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | ascorbic acid | uncompetitive inhibition, 70.3% inhibition at 30 mM | Solanum lycocarpum |  |
| 1.14.18.1 | Citric acid | uncompetitive inhibition, 74.5% inhibition at 10 mM | Solanum lycocarpum | |
| 1.14.18.1 | L-cysteine | competitive inhibition, 74.5% inhibition at 10 mM | Solanum lycocarpum | |
| 1.14.18.1 | Sodium metabisulfite | uncompetitive inhibition, 70.5% inhibition at 10 mM | Solanum lycocarpum | |
| 1.14.18.1 | Thiourea | uncompetitive inhibition, 46% inhibition at 15 mM | Solanum lycocarpum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | additional information | - |
additional information | Michaelis-Menten kinetics | Solanum lycocarpum | |
| 1.14.18.1 | 0.15 | - |
4-methylcatechol | pH 6.5, 28°C | Solanum lycocarpum | |
| 1.14.18.1 | 6.47 | - |
catechol | pH 6.5, 28°C | Solanum lycocarpum | |
| 1.14.18.1 | 20.11 | - |
pyrogallol | pH 6.5, 28°C | Solanum lycocarpum | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 2 4-methylcatechol + O2 | Solanum lycocarpum | - |
2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | Solanum lycocarpum | - |
2 1,2-benzoquinone + 2 H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Solanum lycocarpum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.18.1 | native enzyme, purification procedure is based on freezing precipitation, and a sixfold, partial purification factor is obtained | Solanum lycocarpum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | fruit | unripe fruits present higher activity than ripe for both fresh fruits and dried pulp flours | Solanum lycocarpum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | additional information | - |
172 U/g dry weight and 215 U/g dry weight in unripe and ripe fruits, respectively | Solanum lycocarpum |
| 1.14.18.1 | 1.78 | - |
partially purified enzyme, pH 6.5, 28°C, substrate phenol | Solanum lycocarpum |
| 1.14.18.1 | 3.82 | - |
partially purified enzyme, pH 6.5, 28°C, substrate pyrogallol | Solanum lycocarpum |
| 1.14.18.1 | 20.97 | - |
partially purified enzyme, pH 6.5, 28°C, substrate catechol | Solanum lycocarpum |
| 1.14.18.1 | 67.18 | - |
partially purified enzyme, pH 6.5, 28°C, substrate 4-methylcatechol | Solanum lycocarpum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 2 4-methylcatechol + O2 | - |
Solanum lycocarpum | 2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 4-methylcatechol + O2 | best substrate | Solanum lycocarpum | 2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 catechol + O2 | - |
Solanum lycocarpum | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 phenol + O2 | - |
Solanum lycocarpum | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | additional information | the enzyme is classified as a catecholase type polyphenol oxidase | Solanum lycocarpum | ? | - |
? | |
| 1.14.18.1 | pyrogallol + O2 | - |
Solanum lycocarpum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | ? | x * 47000 + x * 68000, SDS-PAGE | Solanum lycocarpum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | polyphenol oxidase | - |
Solanum lycocarpum |
| 1.14.18.1 | PPO | - |
Solanum lycocarpum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 28 | - |
- |
Solanum lycocarpum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | additional information | - |
results from kinetic and thermodynamic parameters for the thermal inactivation evidence that the partially purified PPO is a biocatalyst, whose inactivation process is related to aggregation of partially unfolded enzyme molecules | Solanum lycocarpum |
| 1.14.18.1 | 30 | - |
partially purified enzyme, half-life is 2.71 h | Solanum lycocarpum |
| 1.14.18.1 | 40 | - |
partially purified enzyme, half-life is 2.03 h | Solanum lycocarpum |
| 1.14.18.1 | 50 | - |
partially purified enzyme, half-life is 1.82 h | Solanum lycocarpum |
| 1.14.18.1 | 60 | - |
partially purified enzyme, half-life is 1.51 h | Solanum lycocarpum |
| 1.14.18.1 | 65 | - |
partially purified enzyme, half-life is 0.80 h | Solanum lycocarpum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 6 | 6.5 | - |
Solanum lycocarpum |
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