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Literature summary extracted from

  • Park, S.B.; Chun, J.H.; Ban, Y.W.; Han, J.Y.; Choi, Y.E.
    Alteration of Panax ginseng saponin composition by overexpression and RNA interference of the protopanaxadiol 6-hydroxylase gene (CYP716A53v2) (2016), J. Ginseng Res., 40, 47-54 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.121 gene CYP716A53v2, recombinant overexpression in Panx ginseng roots using the Agrobacterium tumefaciens strain GV3101 transformation method, expression under control of CaMV 35S promoter, subcloning in Escherichia coli strain DH5alpha Panax ginseng

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.121 additional information construction of transgenic Panax ginseng lines overexpressing CYP716A53v2 gene or silencing CYP716A53v2 gene via RNA interference (RNAi), analyis changes in ginsenoside profiles, overview. Production of protopanaxadiol- and protopanaxatriol-group ginsenosides can be altered by changing the expression of CYP716A53v2 in transgenic Panax ginseng. Changing the expression of CYP716A53v2 affects the downstream genes (glycosyltransferases) Panax ginseng

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.121 protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2 Panax ginseng
-
 protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.121 Panax ginseng I7CT85
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.121 root
-
 Panax ginseng
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.121 protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
-
 Panax ginseng protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.121 CYP716A53v2
-
 Panax ginseng

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.121 cytochrome P450
-
 Panax ginseng
1.14.14.121 NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Panax ginseng

General Information

EC Number General Information Comment Organism
1.14.14.121 metabolism two cytochrome P450 (CYP) genes, CYP716A47 and CYP716A53v2, are critical for the production of protopanaxadiol and protopanaxatriol aglycones, respectively. CYP716A53v2 is a protopanaxadiol 6-hydroxylase that catalyzes protopanaxatriol production from protopanaxadiol in Panax ginseng, biosynthetic pathway for ginsenosides in Panax ginseng, overview Panax ginseng