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Literature summary extracted from

  • Sugimoto, H.; Nakamura, K.; Nishino, Y.; Idezawa, Y.; Fujinuma, A.; Suzuki, K.; Watanabe, T.
    Differences in the roles of the two surface-exposed tyrosine residues, Y240 and Y481, of Serratia marcescens chitinase B during processive degradation of crystalline chitin (2016), J. Gen. Appl. Microbiol., 61, 255-261 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.200 Y240W the mutant retains its substrate-binding ability but shows significantly decreased hydrolytic activity against crystalline beta-chitin compared to the wild type enzyme Serratia marcescens
3.2.1.200 Y240W/Y481W the mutant retains its substrate-binding ability but shows significantly decreased hydrolytic activity against crystalline beta-chitin compared to the wild type enzyme Serratia marcescens
3.2.1.200 Y481W the mutant retains its substrate-binding ability and shows wild type hydrolytic activity against crystalline beta-chitin Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.200 Serratia marcescens P11797
-
-
3.2.1.200 Serratia marcescens 2170 P11797
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.200 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
-
 Serratia marcescens ?
-
 ?
3.2.1.200 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
-
 Serratia marcescens 2170 ?
-
 ?
3.2.1.200 crystalline beta-chitin + H2O the enzyme degrades the chitin from the non-reducing end Serratia marcescens N,N'-diacetylchitobiose + ?
-
 ?
3.2.1.200 crystalline beta-chitin + H2O the enzyme degrades the chitin from the non-reducing end Serratia marcescens 2170 N,N'-diacetylchitobiose + ?
-
 ?
3.2.1.200 glycol chitin + H2O
-
 Serratia marcescens ?
-
 ?
3.2.1.200 glycol chitin + H2O
-
 Serratia marcescens 2170 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.200 ? x * 50000, SDS-PAGE Serratia marcescens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.200 ChiB
-
 Serratia marcescens
3.2.1.200 chitinase B
-
 Serratia marcescens