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Literature summary extracted from
- Purification and characterization of polyphenol oxidase from the bud of Lonicera confusa (2014), J. Food Biochem., 38, 533-541 .
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | ascorbic acid | strong inhibitor, 78% inhibition at 1 mM, 88% inhibition at 10 mM | Lonicera confusa |  |
| 1.14.18.1 | Citric acid | 12% inhibition at 1 mM, 60% inhibition at 10 mM | Lonicera confusa | |
| 1.14.18.1 | Cu2+ | 52% inhibition at 1 mM, 58% inhibition at 10 mM | Lonicera confusa | |
| 1.14.18.1 | EDTA | no inhibition at 1 mM, 17% inhibition at 10 mM | Lonicera confusa | |
| 1.14.18.1 | glutathione | 52% inhibition at 1 mM, 56% inhibition at 10 mM | Lonicera confusa | |
| 1.14.18.1 | kojic acid | 55% inhibition at 1 mM, 86% inhibition at 10 mM | Lonicera confusa | |
| 1.14.18.1 | additional information | poor inhibition by sodium chloride at 10 mM | Lonicera confusa | |
| 1.14.18.1 | Sodium metabisulfite | 67% inhibition at 1 mM, 70% inhibition at 10 mM | Lonicera confusa | |
| 1.14.18.1 | sodium sulfite | 66% inhibition at 1 mM, 70% inhibition at 10 mM | Lonicera confusa | |
| 1.14.18.1 | tropolone | 48% inhibition at 1 mM, 64% inhibition at 10 mM | Lonicera confusa | |
| 1.14.18.1 | Zn2+ | activates by 6% at 5 mM and inhibits by 34% at 10 mM | Lonicera confusa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | additional information | - |
additional information | Michaeli-Menten kinetics | Lonicera confusa | |
| 1.14.18.1 | 0.037 | - |
caffeic acid | pH 8.5, 15°C | Lonicera confusa | |
| 1.14.18.1 | 0.071 | - |
gallate | pH 8.5, 15°C | Lonicera confusa | |
| 1.14.18.1 | 0.11 | - |
pyrogallate | pH 8.5, 15°C | Lonicera confusa | |
| 1.14.18.1 | 0.13 | - |
methylgallate | pH 8.5, 15°C | Lonicera confusa | |
| 1.14.18.1 | 0.28 | - |
catechol | pH 8.5, 15°C | Lonicera confusa | |
| 1.14.18.1 | 2.26 | - |
L-Dopa | pH 8.5, 15°C | Lonicera confusa | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | Ca2+ | activates by 34% at 5 mM and 45% at 10 mM | Lonicera confusa | |
| 1.14.18.1 | Cu2+ | a copper-containing enzyme, exogenous Cu2+ inhibits the enzyme by avbout 55% at 1-10 mM | Lonicera confusa | |
| 1.14.18.1 | Mg2+ | activates by 46% at 5 mM and 59% at 10 mM | Lonicera confusa | |
| 1.14.18.1 | Mn2+ | activates by 76% at 5 mM and 101% at 10 mM | Lonicera confusa | |
| 1.14.18.1 | Zn2+ | activates by 6% at 5 mM and inhibits by 34% at 10 mM | Lonicera confusa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 28800 | - |
gel filtration combined with multiangle laser light scattering | Lonicera confusa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 2 catechol + O2 | Lonicera confusa | - |
2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | Lonicera confusa | - |
2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 pyrocatechol + O2 | Lonicera confusa | - |
? | - |
? | |
| 1.14.18.1 | L-tyrosine + O2 | Lonicera confusa | - |
dopaquinone + H2O | - |
? | |
| 1.14.18.1 | additional information | Lonicera confusa | the PPO from Lonicera confusa exhibits both diphenolase and triphenolase activities, substrates monophenol (L-tyrosine), diphenols (L-DOPA, catechol, caffeic acid) and triphenols (pyrogallic acid, methyl gallate and gallic acid) are used by the enzyme | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Lonicera confusa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.18.1 | native enzyme from flower buds 20.27fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography | Lonicera confusa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | flower bud | - |
Lonicera confusa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 111.75 | - |
crude enzyme extract, pH 8.5, 15°C | Lonicera confusa |
| 1.14.18.1 | 2847.22 | - |
purified enzyme, pH 8.5, 15°C | Lonicera confusa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 2 catechol + O2 | - |
Lonicera confusa | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 L-dopa + O2 | - |
Lonicera confusa | 2 dopaquinone + 2 H2O | - |
? | |
| 1.14.18.1 | 2 pyrocatechol + O2 | - |
Lonicera confusa | ? | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | - |
Lonicera confusa | ? + 2 H2O | - |
? | |
| 1.14.18.1 | gallate + O2 | - |
Lonicera confusa | ? + 2 H2O | - |
? | |
| 1.14.18.1 | L-tyrosine + O2 | - |
Lonicera confusa | dopaquinone + H2O | - |
? | |
| 1.14.18.1 | methylgallate + O2 | - |
Lonicera confusa | ? + 2 H2O | - |
? | |
| 1.14.18.1 | additional information | the PPO from Lonicera confusa exhibits both diphenolase and triphenolase activities, substrates monophenol (L-tyrosine), diphenols (L-DOPA, catechol, caffeic acid) and triphenols (pyrogallic acid, methyl gallate and gallic acid) are used by the enzyme | Lonicera confusa | ? | - |
? | |
| 1.14.18.1 | pyrogallate + O2 | - |
Lonicera confusa | ? + 2 H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | monomer | 1 * 28800, SDS-PAGE | Lonicera confusa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | polyphenol oxidase | - |
Lonicera confusa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 15 | - |
- |
Lonicera confusa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 10 | 20 | purified native enzyme, 60 min, over 90% activity remaining | Lonicera confusa |
| 1.14.18.1 | 40 | 60 | purified native enzyme, 60 min, loss of 50% activity | Lonicera confusa |
| 1.14.18.1 | 60 | - |
purified native enzyme, 10 min, loss of 30% activity | Lonicera confusa |
| 1.14.18.1 | 70 | 80 | purified native enzyme, 10 min, loss of over 70% activity, inactivation | Lonicera confusa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 8.5 | - |
- |
Lonicera confusa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 6.5 | 8.5 | at pH below 6.0, PPO is inactivated. The PPO activity rapidly increases with pH in the range of 6.5-8.5, above pH 8.5, the activity decreases to about 80% of maximal activity at pH 9.0-10.0 | Lonicera confusa |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 8 | 10 | more than 80% of maximal activity is maintained between pH 9.0 and pH 10.0, high activity in the pH range of 8.0-10.0 | Lonicera confusa |
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