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Literature summary extracted from

  • Shaw, J.; Harayama, S.
    Characterization in vitro of the hydroxylase component of xylene monooxygenase, the first enzyme of the TOL-plasmid-encoded pathway for the mineralization of toluene and xylenes (1995), J. Ferment. Bioeng., 79, 195-199 .
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.14.15.26 after separation of the solubilized enzyme by anion exchange chromatography, the stability of xylene monooxygenase is drastically reduced Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.26 membrane
-
 Pseudomonas putida 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.26 Fe2+ required Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.26 Pseudomonas putida P21395 encoded by TOL plasmid
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.26 activity can be solubilized by detergents, octyl-beta-glucopyranoside and 3-[(3-cholamidopropyl)dimethyiammonio]-l-propanesulfonate Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.26 m-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 3-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 o-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 2-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 p-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 toluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida benzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.26 7
-
-
 Pseudomonas putida