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Literature summary extracted from

  • Cho, S.H.; Kang, K.; Lee, S.H.; Lee, I.J.; Paek, N.C.
    OsWOX3A is involved in negative feedback regulation of the gibberellic acid biosynthetic pathway in rice (Oryza sativa) (2016), J. Exp. Bot., 67, 1677-1687 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.107 gene KAO encoding ent-kaurenoic acid oxidase, quantitative RT-PCR enzyme expression analysis Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.107 ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 Oryza sativa Japonica Group overall reaction gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
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Organism

EC Number Organism UniProt Comment Textmining
1.14.14.107 Oryza sativa Japonica Group Q33DK1 cv. Dongjinbye
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.107 additional information KAO is broadly expressed in many organs and tissues Oryza sativa Japonica Group
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.107 ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Oryza sativa Japonica Group gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
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Synonyms

EC Number Synonyms Comment Organism
1.14.14.107 KAO
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 Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.107 cytochrome P-450
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 Oryza sativa Japonica Group
1.14.14.107 NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
1.14.14.107 Oryza sativa Japonica Group OsWOX3A interacts directly with the KAO promoter to repress KAO expression. OsWOX3A interacts with a WOX-binding motif, TTAATCG, in the KAO promoter, mechanism modeling, overview. OsWOX3A is involved in the negative feedback regulation of KAO expression for gibberellic acid homeostasis down

General Information

EC Number General Information Comment Organism
1.14.14.107 malfunction the rice mutant of KAO (oskao-1) has low levels of GA53, GA20, and GA1, OsWOX3A-OX downregulates KAO expression, which causes significantly reduced levels of GA53, GA20, and, finally, bioactive GA1. Quantitative analysis of gibberellic acid intermediates reveals significantly reduced levels of GA20 and bioactive GA1 in transgenic rice overexpressing OsWOX3A-OX, due to downregulation of the expression of KAO. The mutant OsWOX3A-OX exhibits severe dwarfism with very short and wide leaves, a phenotype that resembles that of gibberellic acid (GA)-deficient or GA-insensitive mutants. Loss of OsWOX3A function in narrow leaf2 (nal2) nal3 double mutants (termed nal2/3) causes pleiotropic effects, such as narrow and curly leaves, opened spikelets, narrow grains, more tillers, and fewer lateral roots, but almost normal plant height. Loss-of-function nal2/3 mutants show increased expression of KAO, changes in gibberellic acid contents are consistent with the expression levels of KAO and GA20ox2 in nal2/3 mutants Oryza sativa Japonica Group
1.14.14.107 additional information OsWOX3A acts as a transcriptional repressor, generation of transgenic rice overexpressing OsWOX3A, OsWOX3A-OX, the mutant consistently exhibits severe dwarfism with very short and wide leaves, a phenotype that resembles that of gibberellic acid (GA)-deficient or GA-insensitive mutants. OsWOX3A expression is drastically and temporarily upregulated by gibberellin GA3 and is downregulated by paclobutrazol, a blocker of gibberellic acid biosynthesis Oryza sativa Japonica Group
1.14.14.107 physiological function ent-kaurenoic acid oxidase, encoded by gene KAO, is a gibberellin biosynthetic enzyme. WUSCHEL-related homeobox 3 in Oryza sativa, OsWOX3A, is a gibberellic acid-responsive gene and functions in the negative feedback regulation of the gibberellic acid biosynthetic pathway for gibberellic acid homeostasis to maintain the threshold levels of endogenous gibberellic acid intermediates throughout development. OsWOX3A interacts directly with the KAO promoter to repress KAO expression. Changes in gibberellic acid contents are consistent with the expression levels of KAO and GA20ox2 in nal2/3 mutants. OsWOX3A directly downregulates the expression of KAO as a trans-repressor or upregulates the expression of GA20ox2 as a trans-activator Oryza sativa Japonica Group