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Literature summary extracted from

  • Kaneshiro, E.; Johnston, L.; Nkinin, S.; Romero, B.; Giner, J.
    Sterols of Saccharomyces cerevisiae erg6 knockout mutant expressing the Pneumocystis carinii S-adenosylmethionine sterol C-24 methyltransferase (SAM SMT) (2015), J. Eukaryot. Microbiol., 62, 298-306 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.71 gene erg6, recombinant expression in transgenic Saccharomyces cerevisiae erg6 knockout mutant strain and functional complementation Pneumocystis carinii

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.71 additional information analysis of sterol compositions of wild-type Sacchromyces cerevisiae strain BY4742, the erg6 knockout mutant (DELTAerg6), and DELTAerg6 expressing the Pneumocystis carinii or the Saccharomyces cerevisiae erg6 gene by a variety of chromatographic and spectroscopic procedures. The Pneumocystis carinii SAM:SMT in the DELTAerg6 restores its ability to produce the C28 sterol ergosterol as the major sterol, and also results in low levels of C29 sterols, overview. While the Pneumocystis carinii SAM:SMT in the yeast DELTAerg6 cells is able to transfer a second methyl group to the side chain, the action of DELTA24(28)-sterol reductase (coded by the erg4 gene) in the yeast cells prevents the formation and accumulation of as many C29 sterols as that found in Pneumocystis carinii Pneumocystis carinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.71 ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+ Pneumocystis carinii
-
 ergosterol + NADP+
-
 ?
1.3.1.71 ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+ Pneumocystis carinii B80
-
 ergosterol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.71 Pneumocystis carinii A0A0W4ZCX9
-
-
1.3.1.71 Pneumocystis carinii B80 A0A0W4ZCX9
-
-
2.1.1.41 Pneumocystis carinii Q96WX4 catalyzes the reactions of both EC 2.1.1.142 and EC 2.1.1.41
-
2.1.1.41 Pneumocystis carinii B80 Q96WX4 catalyzes the reactions of both EC 2.1.1.142 and EC 2.1.1.41
-
2.1.1.142 Pneumocystis carinii Q96WX4 enzyme catalyzes both the reactions of EC 2.1.1.41 and EC 2.1.1.142
-
2.1.1.142 Pneumocystis carinii B80 Q96WX4 enzyme catalyzes both the reactions of EC 2.1.1.41 and EC 2.1.1.142
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.71 ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
-
 Pneumocystis carinii ergosterol + NADP+
-
 ?
1.3.1.71 ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
-
 Pneumocystis carinii B80 ergosterol + NADP+
-
 ?
1.3.1.71 additional information the enzyme transfers either one or two methyl groups to the C-24 position of the sterol side chain producing both C28 and C29 24-alkylsterols in approximately the same proportions whereas most fungal SAM:SMT transfer only one methyl group to the side chain Pneumocystis carinii ?
-
 ?
1.3.1.71 additional information the enzyme transfers either one or two methyl groups to the C-24 position of the sterol side chain producing both C28 and C29 24-alkylsterols in approximately the same proportions whereas most fungal SAM:SMT transfer only one methyl group to the side chain Pneumocystis carinii B80 ?
-
 ?
2.1.1.142 S-adenosyl-L-methionine + lanosterol
-
 Pneumocystis carinii S-adenosyl-L-homocysteine + 24(28)-methylene-24,25-dihydro-lanosterol
-
 ?
2.1.1.142 S-adenosyl-L-methionine + lanosterol
-
 Pneumocystis carinii B80 S-adenosyl-L-homocysteine + 24(28)-methylene-24,25-dihydro-lanosterol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.41 ? x * 43000, SDS-PAGE Pneumocystis carinii
2.1.1.142 ? x * 43000, SDS-PAGE Pneumocystis carinii

Synonyms

EC Number Synonyms Comment Organism
1.3.1.71 Erg6
-
 Pneumocystis carinii
1.3.1.71 S-adenosylmethionine:sterol C-24 methyltransferase
-
 Pneumocystis carinii
1.3.1.71 SAM:SMT
-
 Pneumocystis carinii
2.1.1.41 Erg6
-
 Pneumocystis carinii
2.1.1.41 SAM:SMT
-
 Pneumocystis carinii
2.1.1.142 Erg6
-
 Pneumocystis carinii
2.1.1.142 SAM:SMT
-
 Pneumocystis carinii

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.71 NADPH
-
 Pneumocystis carinii

General Information

EC Number General Information Comment Organism
2.1.1.41 physiological function enzyme transfers either one or two methyl groups to the C-24 position of the sterol side chain producing both C28 and C29 24-alkylsterols in approximately the same proportions. Expression of SAM:SMT in a Saccharomyces cerevisiae Erg6 mutant restores its ability to produce ergosterol as the major sterol, and also results in low levels of C29 sterols such as pneumocysterol Pneumocystis carinii
2.1.1.142 physiological function enzyme transfers either one or two methyl groups to the C-24 position of the sterol side chain producing both C28 and C29 24-alkylsterols in approximately the same proportions. Expression of SAM:SMT in a Saccharomyces cerevisiae Erg6 mutant restores its ability to produce ergosterol as the major sterol, and also results in low levels of C29 sterols such as pneumocysterol Pneumocystis carinii