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Literature summary extracted from

  • Nguyen, D.M.N.; Schut, G.J.; Zadvornyy, O.A.; Tokmina-Lukaszewska, M.; Poudel, S.; Lipscomb, G.L.; Adams, L.A.; Dinsmore, J.T.; Nixon, W.J.; Boyd, E.S.; Bothner, B.; Peters, J.W.; Adams, M.W.W.
    Two functionally distinct NADP+-dependent ferredoxin oxidoreductases maintain the primary redox balance of Pyrococcus furiosus (2017), J. Biol. Chem., 292, 14603-14616 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.18.1.2 vapor diffusion method, using 0.22 M magnesium sulfate, 27% (w/v) polyethylene glycol 3350, and 0.4% (v/v) ethyl acetate in the presence of 1 mM sodium dithionite Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 88951
-
 mass spectrometry Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.2 reduced ferredoxin + NADP+ + H+ Pyrococcus furiosus
-
 oxidized ferredoxin + NADPH
-
 ?
1.18.1.2 reduced ferredoxin + NADP+ + H+ Pyrococcus furiosus COM1
-
 oxidized ferredoxin + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.4 Pyrococcus furiosus Q8U195 and Q8U194 Q8U195: subunit alpha, Q8U194: subunit beta
-
1.18.1.2 Pyrococcus furiosus I6TYZ3
-
-
1.18.1.2 Pyrococcus furiosus COM1 I6TYZ3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 His-Trap column chromatography, Ni-NTA column chromatography, and S100 gel filtration Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.2 17
-
 after 1fold purification, at pH 7.5 and 80°C Pyrococcus furiosus
1.18.1.2 275
-
 after 15fold purification, at pH 7.5 and 80°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.1.4 NADH + H+ + 2 NADP+ + 2 reduced ferredoxin [iron-sulfur] cluster
-
 Pyrococcus furiosus NAD+ + 2 NADPH + 2 oxidized ferredoxin [iron-sulfur] cluster
-
 ?
1.18.1.2 additional information the enzyme does not perform NADH-dependent benzyl viologen reduction Pyrococcus furiosus ?
-
 ?
1.18.1.2 additional information the enzyme does not perform NADH-dependent benzyl viologen reduction Pyrococcus furiosus COM1 ?
-
 ?
1.18.1.2 reduced benzyl viologen + NADP+ + H+
-
 Pyrococcus furiosus oxidized benzyl viologen + NADPH
-
 ?
1.18.1.2 reduced benzyl viologen + NADP+ + H+
-
 Pyrococcus furiosus COM1 oxidized benzyl viologen + NADPH
-
 ?
1.18.1.2 reduced ferredoxin + NADP+ + H+
-
 Pyrococcus furiosus oxidized ferredoxin + NADPH
-
 ?
1.18.1.2 reduced ferredoxin + NADP+ + H+
-
 Pyrococcus furiosus COM1 oxidized ferredoxin + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.1.2 heterodimer 1 * 52498 + 1 * 34029, calculated from amino acid sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.6.1.4 NADH-dependent ferredoxin NADP+ oxidoreductase I
-
 Pyrococcus furiosus
1.6.1.4 Nfn1
-
 Pyrococcus furiosus
1.6.1.4 PF1327-28
-
 Pyrococcus furiosus
1.18.1.2 ferredoxin NADP oxidoreductase
-
 Pyrococcus furiosus
1.18.1.2 NfnIII
-
 Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.1.4 flavin flavoprotein Pyrococcus furiosus
1.18.1.2 FAD
-
 Pyrococcus furiosus
1.18.1.2 NADP+
-
 Pyrococcus furiosus
1.18.1.2 [2Fe-2S]-center
-
 Pyrococcus furiosus
1.18.1.2 [4Fe-4S]-center
-
 Pyrococcus furiosus

General Information

EC Number General Information Comment Organism
1.6.1.4 metabolism the enzyme has a key role in maintaining redox homeostasis. It is involved in maintaining the cellular redox balance, producing NADPH for biosynthesis by recycling the two other primary redox carriers, NADH and ferredoxin. The enzyme effectively couples the endergonic reduction of NADP+ by NADH and the exergonic reduction of NADP+ by reduced ferredoxin, thereby maintaining a high ratio of NADPH/NADP+ to drive biosynthesis. The Pyrococcus furiosus genome encodes an NfnI paralog termed NfnII, and the two are differentially expressed, depending on the growth conditions. Deletion of the genes encoding either NfnI or NfnII affects the cellular concentrations of NAD(P)H and particularly NADPH. This results in a moderate to severe growth phenotype in deletion mutants, demonstrating a key role for each enzyme in maintaining redox homeostasis Pyrococcus furiosus
1.18.1.2 physiological function the enzyme is important for growth in the presence of sulfur Pyrococcus furiosus