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Literature summary extracted from

  • Makhlynets, O.; Boal, A.; Rhodes, D.; Kitten, T.; Rosenzweig, A.; Stubbe, J.
    Streptococcus sanguinis class Ib ribonucleotide reductase High activity with both iron and manganese cofactors and structural insights (2014), J. Biol. Chem., 289, 6259-6272 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.17.4.1 drug development class Ib NrdF requires manganese under conditions in which the organism is pathogenic. Considering that streptococci and other pathogens, including enterococci, staphylococci, and Bacillus sp., contain class Ib ribonucleotide reductase as their only aerobic RNR, prevention of dimanganese-tyrosyl radical (Mn(III)2-Y(*)) cofactor formation in the class Ib ribonucleotide reductase may be an attractive target for new antimicrobials Streptococcus sanguinis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.4.1 expression in Escherichia coli BL21(DE3) cells Streptococcus sanguinis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Fe3+ the dimanganese-tyrosyl radical (Mn(III)2-Y(*)) cofactor is 3.5fold more active than the iron form Streptococcus sanguinis
1.17.4.1 Mn3+ the enzyme uses a dimanganese-tyrosyl radical (Mn(III)2-Y(*)) cofactor in vivo Streptococcus sanguinis

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Streptococcus sanguinis A3CLZ6 AND A3CLZ4 A3CLZ6: subunit alpha (NrdE), A3CLZ4: subunit beta (NrdF)
-
1.17.4.1 Streptococcus sanguinis SK36 A3CLZ6 AND A3CLZ4 A3CLZ6: subunit alpha (NrdE), A3CLZ4: subunit beta (NrdF)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1
-
 Streptococcus sanguinis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 CDP + thioredoxin
-
 Streptococcus sanguinis 2'-dCDP + thioredoxin disulfide + H2O
-
 ?
1.17.4.1 CDP + thioredoxin
-
 Streptococcus sanguinis SK36 2'-dCDP + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.4.1 class Ib ribonucleotide reductase
-
 Streptococcus sanguinis
1.17.4.1 class Ib RNR
-
 Streptococcus sanguinis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.1 37
-
 assay at Streptococcus sanguinis

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.1 diferric(III)-tyrosyl radical cofactor the dimanganese-tyrosyl radical (Mn(III)2-Y(*)) cofactor is 3.5fold more active than the iron form. The flavodoxin NrdI is essential for assembly of the ribonucleotide reductase metallo-cofactor Streptococcus sanguinis
1.17.4.1 dimanganese-tyrosyl radical cofactor the enzyme uses a dimanganese-tyrosyl radical (Mn(III)2-Y(*)) cofactor in vivo. The dimanganese-tyrosyl radical (Mn(III)2-Y(*)) cofactor is 3.5fold more active than the iron form. The flavodoxin NrdI is essential for assembly of the ribonucleotide reductase metallo-cofactor Streptococcus sanguinis