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Literature summary extracted from

  • Tsukamoto, H.; Takakura, Y.; Yamamoto, T.
    Purification, cloning, and expression of an alpha/beta-galactoside alpha-2,3-sialyltransferase from a luminous marine bacterium, Photobacterium phosphoreum (2007), J. Biol. Chem., 282, 29794-29802 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.6 expression of full-length and truncated protein in Escherichia coli Photobacterium phosphoreum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.6 0.05
-
 CMP-N-acetyl-beta-neuraminate pH 6.0, 25°C Photobacterium phosphoreum
2.4.3.6 1.7
-
 beta-D-Gal-(1->4)-beta-D-Glc pH 6.0, 25°C Photobacterium phosphoreum
2.4.3.6 2.5
-
 beta-D-Gal-(1->4)-beta-D-GlcNAc pH 6.0, 25°C Photobacterium phosphoreum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.3.6 NaCl native and recombinant enzymes are twice as active in the presence of NaCl at 500, 750, and 1000 mM as in the absence of NaCl Photobacterium phosphoreum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.6 40000
-
 SDS-PAGE Photobacterium phosphoreum
2.4.3.6 45026
-
 MALDI-TOF MS analysis Photobacterium phosphoreum

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.6 Photobacterium phosphoreum A5LHX0
-
-
2.4.3.6 Photobacterium phosphoreum JT-ISH-467 A5LHX0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.6
-
 Photobacterium phosphoreum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-Glc
-
 Photobacterium phosphoreum CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-Glc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-Glc
-
 Photobacterium phosphoreum JT-ISH-467 CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-Glc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-GlcNAc 103% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme Photobacterium phosphoreum CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-GlcNAc 103% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme Photobacterium phosphoreum JT-ISH-467 CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-mannopyranoside 43% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme Photobacterium phosphoreum CMP + alpha-Neu5Ac-(2->3)-beta-D-Man
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-mannopyranoside 43% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme Photobacterium phosphoreum JT-ISH-467 CMP + alpha-Neu5Ac-(2->3)-beta-D-Man
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-N-acetylglucosamine 40% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme Photobacterium phosphoreum CMP + alpha-Neu5Ac-(2->3)-beta-D-GlcNAc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-N-acetylglucosamine 40% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme Photobacterium phosphoreum JT-ISH-467 CMP + alpha-Neu5Ac-(2->3)-beta-D-GlcNAc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + methyl beta-D-Gal-(1->4)-beta-D-GlcNAc 129% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme Photobacterium phosphoreum CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + methyl beta-D-Gal-(1->4)-beta-D-GlcNAc 129% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme Photobacterium phosphoreum JT-ISH-467 CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + methyl beta-D-galactopyranoside 251% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme Photobacterium phosphoreum CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Gal
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + methyl beta-D-glucopyranoside 33% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme Photobacterium phosphoreum CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Glc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + methyl beta-D-mannopyranoside 63% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, truncated recombinant enzyme Photobacterium phosphoreum CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Man
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.6 ? x * 40000, SDS-PAGE Photobacterium phosphoreum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.6 20 25 native enzyme and the full-length recombinant enzyme Photobacterium phosphoreum
2.4.3.6 25 30 truncated recombinant enzyme Photobacterium phosphoreum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.6 5 5.5 native enzyme and the full-length recombinant enzyme Photobacterium phosphoreum
2.4.3.6 5.5 6 truncated recombinant enzyme Photobacterium phosphoreum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.3.6 5 11 active between pH 5.0 and pH 11.0, native enzyme and recombinant enzyme Photobacterium phosphoreum