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Literature summary extracted from

  • Tsukamoto, H.; Takakura, Y.; Mine, T.; Yamamoto, T.
    Photobacterium sp. JT-ISH-224 produces two sialyltransferases, alpha-/beta-galactoside alpha2,3-sialyltransferase and beta-galactoside alpha2,6-sialyltransferase (2008), J. Biochem., 143, 187-197 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.6 expression in Escherichia coli Photobacterium sp. JT-ISH-224

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.6 31
-
 beta-D-Gal-(1->4)-beta-D-Glc pH 6.0, 30°C Photobacterium sp. JT-ISH-224
2.4.3.6 99
-
 methyl beta-D-galactopyranoside pH 6.0, 30°C Photobacterium sp. JT-ISH-224

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.6 56732
-
 MALDI-TOF Photobacterium sp. JT-ISH-224

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.6 Photobacterium sp. JT-ISH-224 A8QYL0
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.6 113
-
 pH 6.0, 30°C Photobacterium sp. JT-ISH-224

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-Glc
-
 Photobacterium sp. JT-ISH-224 CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-Glc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-GlcNAc 96% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, recombinant enzyme Photobacterium sp. JT-ISH-224 CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-N-acetylgalactosamine 76% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, recombinant enzyme Photobacterium sp. JT-ISH-224 CMP + alpha-Neu5Ac-(2->3)-beta-D-GalNAc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + methyl beta-D-Gal-(1->3)-beta-D-GlcNAc 192% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, recombinant enzyme Photobacterium sp. JT-ISH-224 CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + methyl beta-D-galactopyranoside 343% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, recombinant enzyme Photobacterium sp. JT-ISH-224 CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-galactopyranoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.6 ? x * 56732, MALDI-TOF Photobacterium sp. JT-ISH-224

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.6 25 30
-
 Photobacterium sp. JT-ISH-224

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.6 5 5.5
-
 Photobacterium sp. JT-ISH-224