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Literature summary extracted from

  • Kondadi, P.K.; Rossi, M.; Twelkmeyer, B.; Schur, M.J.; Li, J.; Schott, T.; Paulin, L.; Auvinen, P.; Haenninen, M.L.; Schweda, E.K.; Wakarchuk, W.
    Identification and characterization of a lipopolysaccharide alpha,2,3-sialyltransferase from the human pathogen Helicobacter bizzozeronii (2012), J. Bacteriol., 194, 2540-2550 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.6 expressed in Escherichia coli AD202 cells Helicobacter bizzozeronii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.6 0.1085
-
 FCHASE beta-D-Gal-(1->4)-beta-D-GlcNAc pH and temperature not specified in the publication Helicobacter bizzozeronii
2.4.3.6 0.1255
-
 CMP-N-acetyl-beta-neuraminate pH and temperature not specified in the publication Helicobacter bizzozeronii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.6 80000
-
 x * 80000, fusion protein, SDS-PAGE Helicobacter bizzozeronii

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.6 Helicobacter bizzozeronii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.6
-
 Helicobacter bizzozeronii

Storage Stability

EC Number Storage Stability Organism
2.4.3.6 -70°C, purified enzyme, over 50 days, isoform ST2 remains stable Helicobacter bizzozeronii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-GlcNAc i.e. N-acetyllactosamine. Sialyltransferase Hb-ST2 shows monofunctional alpha2,3-sialyltransferase activity with a strong preference for N-acetyllactosamine over lactose and very low levels of activity on 3-linked Gal. No 2,8-sialyltransferase activity Helicobacter bizzozeronii CMP + alpha-NeuAc-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc
-
 ?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + FCHASE beta-D-Gal-(1->4)-beta-D-GlcNAc
-
 Helicobacter bizzozeronii CMP + FCHASE alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.6 ? x * 80000, fusion protein, SDS-PAGE Helicobacter bizzozeronii

Synonyms

EC Number Synonyms Comment Organism
2.4.3.6 GT-42 sialyltransferase
-
 Helicobacter bizzozeronii
2.4.3.6 Hb-ST2
-
 Helicobacter bizzozeronii