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Literature summary extracted from

  • Eaton, R.W.
    p-Cymene catabolic pathway in Pseudomonas putida F1 cloning and characterization of DNA encoding conversion of p-cymene to p-cumate (1997), J. Bacteriol., 179, 3171-3180 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.25 expression in Escherichia coli Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.25 Pseudomonas putida O33456 and O33457 O33456 i.e. hydroxylase subunit CymAa, O33457 i.e. reductase subunit CymAb, cf. EC 1.8.1.3
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.25 additional information enzyme can attack benzylic and toluic alcohols as well as toluene and xylenes, probably with the formation of gem-diol intermediates which spontaneously dehydrate to yield the corresponding aldehydes Pseudomonas putida ?
-
 ?
1.14.15.25 p-cymene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-isopropylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.25 ? x * 43117, hydrolase subunit CymAa, x * 38295, reductase subunit CymAb, calculated Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.25 Ferredoxin reductase subunit CymAb Pseudomonas putida
1.14.15.25 [2Fe-2S]-center reductase subunit CymAb Pseudomonas putida