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Literature summary extracted from

  • Patil, S.; Sistla, S.; Jadhav, J.
    Screening of inhibitors for mushroom tyrosinase using surface plasmon resonance (2014), J. Agric. Food Chem., 62, 11594-11601 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.18.1 drug development the regulation of melanin synthesis via the inhibition of tyrosinase is a research topic in the context of preventing hyperpigmentation, thus tyrosinase inhibitors are increasingly important in the food, medical, and cosmetic industries. The evaluated suface plasmon resonance biosensor has the potential to be used in the detection and screening of inhibitor drug candidates Agaricus bisporus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 catechol enzyme-inhibitor interaction measurement by SPR Agaricus bisporus
1.14.18.1 additional information inhibitor screening using surface plasmon resonance (SPR), two-state modeling. Structural changes of the mushroom tyrosinase in the presence of inhibitors are analyzed by circular dichroism spectroscopy Agaricus bisporus
1.14.18.1 phloroglucinol enzyme-inhibitor interaction measurement by SPR Agaricus bisporus
1.14.18.1 pyrogallol enzyme-inhibitor interaction measurement by SPR Agaricus bisporus
1.14.18.1 saffron enzyme-inhibitor interaction measurement by SPR Agaricus bisporus
1.14.18.1 Tannic acid enzyme-inhibitor interaction measurement by SPR Agaricus bisporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.1 2 L-dopa + O2 Agaricus bisporus
-
 2 dopaquinone + 2 H2O
-
 ?
1.14.18.1 L-tyrosine + O2 Agaricus bisporus
-
 dopaquinone + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Agaricus bisporus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 commercial preparation
-
 Agaricus bisporus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 2 L-dopa + O2
-
 Agaricus bisporus 2 dopaquinone + 2 H2O
-
 ?
1.14.18.1 L-tyrosine + O2
-
 Agaricus bisporus dopaquinone + H2O
-
 ?
1.14.18.1 additional information monophenolase and diphenolase activities of mushroom tyrosinase are performed using L-tyrosine and L-DOPA, respectively, by measuring the dopachrome accumulation at 475 nm before immobilization on the chip surface for surface plasmon resonance analysis Agaricus bisporus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 mushroom tyrosinase
-
 Agaricus bisporus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.18.1 additional information
-
 additional information inhibition kinetics Agaricus bisporus

General Information

EC Number General Information Comment Organism
1.14.18.1 physiological function tyrosinase is a multifunctional copper-containing oxygenase and catalyzes the hydroxylation of a monophenol and the conversion of o-diphenols to the corresponding o-quinones. These quinones are highly reactive compounds that can polymerize spontaneously to form melanin. Products formed as a result of tyrosinase activity are precursors for skin pigmentation, defense, and protective mechanisms in fungi Agaricus bisporus