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Literature summary extracted from

  • Datta, S.; Holmes, B.; Park, J.; Chen, Z.; Dibble, D.; Hadi, M.; Blanch, H.; Simmons, B.; Sapra, R.
    Ionic liquid tolerant hyperthermophilic cellulases for biomass pretreatment and hydrolysis (2010), Green Chem., 12, 338-345 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.4 biofuel production potential of using the ionic liquids-tolerant extremophilic cellulases for hydrolysis of ionic liquids-pretreated lignocellulosic biomass, for biofuel production Thermotoga maritima
3.2.1.4 biofuel production potential of using the ionic liquids-tolerant extremophilic cellulases for hydrolysis of ionic liquids-pretreated lignocellulosic biomass, for biofuel production Pyrococcus horikoshii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli Thermotoga maritima
3.2.1.4 expression in Escherichia coli Pyrococcus horikoshii

General Stability

EC Number General Stability Organism
3.2.1.4 under optimum conditions the enzyme shows significantly higher ionic liquid 1-ethyl-3-methylimidazolium acetate tolerance than the Trichoderma viride cellulase Thermotoga maritima
3.2.1.4 under optimum conditions the enzyme shows significantly higher ionic liquid 1-ethyl-3-methylimidazolium acetate tolerance than the Trichoderma viride cellulase Pyrococcus horikoshii

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.4 1-ethyl-3-methylimidazolium acetate under optimum conditions the enzyme shows significantly higher 1-ethyl-3-methylimidazolium acetate tolerance than the Trichoderma viride cellulase Thermotoga maritima
3.2.1.4 1-ethyl-3-methylimidazolium acetate under optimum conditions the enzyme shows significantly higher 1-ethyl-3-methylimidazolium acetate tolerance than the Trichoderma viride cellulase Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Pyrococcus horikoshii
-
-
-
3.2.1.4 Pyrococcus horikoshii OT-3
-
-
-
3.2.1.4 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Thermotoga maritima
3.2.1.4
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O
-
 Thermotoga maritima ?
-
 ?
3.2.1.4 avicel + H2O
-
 Pyrococcus horikoshii ?
-
 ?
3.2.1.4 avicel + H2O
-
 Pyrococcus horikoshii OT-3 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Thermotoga maritima ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Pyrococcus horikoshii ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Pyrococcus horikoshii OT-3 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel5A
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 80
-
-
 Thermotoga maritima
3.2.1.4 85
-
 assay at Pyrococcus horikoshii
3.2.1.4 97
-
-
 Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4.8
-
-
 Thermotoga maritima
3.2.1.4 5.6
-
 assay at Pyrococcus horikoshii
3.2.1.4 6.4
-
-
 Pyrococcus horikoshii