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Literature summary extracted from

  • Yasumoto, S.; Seki, H.; Shimizu, Y.; Fukushima, E.O.; Muranaka, T.
    Functional characterization of CYP716 family P450 enzymes in triterpenoid biosynthesis in tomato (2017), Front. Plant Sci., 8, 21 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.126 gene CYP716A44, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, in beta-amyrin synthase (EC 5.4.99.39) and CYP716A44 co-expressing Saccharomyces cerevisiae strains SY57 and SY66 erythrodiol and oleanolic acid are detected as C-28 oxidation product of beta-amyrin Solanum lycopersicum
1.14.14.126 gene CYP716A46, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, in beta-amyrin synthase (EC 5.4.99.39) and CYP716A46 co-expressing Saccharomyces cerevisiae strains SY57 and SY66, erythrodiol and oleanolic acid are detected as C-28 oxidation product of beta-amyrin Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.126 beta-amyrin + 3 O2 + 3 NADPH + 3 H+ Solanum lycopersicum
-
 oleanolate + 3 NADP+ + 4 H2O
-
 ?
1.14.14.126 beta-amyrin + O2 + NADPH + H+ Solanum lycopersicum
-
 erythrodiol + NADP+ + H2O
-
 ?
1.14.14.126 additional information Solanum lycopersicum the enzyme produces erythrodiol and oleanolic acid as C-28 oxidation products of beta-amyrin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.64 Solanum lycopersicum A0A3Q7HS74
-
-
1.14.14.126 Solanum lycopersicum A0A3Q7HBJ5 cv. Micro-Tom
-
1.14.14.126 Solanum lycopersicum K4CEE8 cv. Heinz 1706
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.64 root specifically expressed in root Solanum lycopersicum
-
1.14.14.126 flower exclusive expresion of CYP716A46 in flowers Solanum lycopersicum
-
1.14.14.126 flower very low expresion level Solanum lycopersicum
-
1.14.14.126 additional information no enzyme expression in leaves Solanum lycopersicum
-
1.14.14.126 additional information no enzyme expression in leaves, roots, and stems Solanum lycopersicum
-
1.14.14.126 root high enzyme level Solanum lycopersicum
-
1.14.14.126 stem very low expresion level Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.64 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
 Solanum lycopersicum daturadiol + [oxidized NADPh-hemoprotein reductase] + H2O
-
 ?
1.14.14.64 additional information enzyme shows C-6beta hydroxylation activity against beta-amyrin to produce daturadiol(olean-12-ene-3b,6b-diol) Solanum lycopersicum ?
-
 ?
1.14.14.126 beta-amyrin + 3 O2 + 3 NADPH + 3 H+
-
 Solanum lycopersicum oleanolate + 3 NADP+ + 4 H2O
-
 ?
1.14.14.126 beta-amyrin + O2 + NADPH + H+
-
 Solanum lycopersicum erythrodiol + NADP+ + H2O
-
 ?
1.14.14.126 additional information the enzyme produces erythrodiol and oleanolic acid as C-28 oxidation products of beta-amyrin Solanum lycopersicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.64 CYP716A43
-
 Solanum lycopersicum
1.14.14.64 CYP716E26
-
 Solanum lycopersicum
1.14.14.64 Solyc06g065430
-
 Solanum lycopersicum
1.14.14.126 101251676
-
 Solanum lycopersicum
1.14.14.126 101252702
-
 Solanum lycopersicum
1.14.14.126 CYP716A44
-
 Solanum lycopersicum
1.14.14.126 CYP716A46
-
 Solanum lycopersicum
1.14.14.126 More formerly EC 1.14.13.201 Solanum lycopersicum
1.14.14.126 multifunctional triterpene C-28 oxidase
-
 Solanum lycopersicum

General Information

EC Number General Information Comment Organism
1.14.14.126 evolution the enzyme belongs to the CYP716C subfamily, many CYP716 family P450 enzymes are characterized as multifunctional triterpene C-28 oxidases, which oxidize alpha-amyrin and beta-amyrin to the widely distributed triterpenoids ursolic and oleanolic acids, respectively. Isolation of all six CYP716 family genes from tomato, including CYP716A46, and sequence comparisons Solanum lycopersicum
1.14.14.126 evolution the enzyme belongs to the CYP716C subfamily, many CYP716 family P450 enzymes are characterized as multifunctional triterpene C-28 oxidases, which oxidize alpha-amyrin and beta-amyrin to the widely distributed triterpenoids ursolic and oleanolic acids, respectively. Isolation of all six CYP716 family genes from tomato, including gene CYP716A44, and sequence comparisons Solanum lycopersicum
1.14.14.126 metabolism the enzyme is involved in the triterpenoid biosynthesis in tomato Solanum lycopersicum