EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.45 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, transient expression of GFP-tagged enzyme in Glycine max protoplasts' cytoplasm, recombinant overexpression of the enzyme under the control of a CaMV35S promoter via Agrobacterium tumefaciens strain LBA4404 and tri-parental mating in transgenic Glycine max plants, quantitative real-time PCR enzyme expression analysis, recombinant expression of Nterminally His6-tagged enzyme in Escherichia coli strain Transetta (DE3) | Glycine max |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.1.45 | additional information | enzyme overexpression in Glycine max transgenic plants results in highly reduced daidzein levels, while the relative content of glyceollins in transgenic plants is significantly higher than that of non-transgenic plants. The relative expression levels of reactive oxygen species (ROS) of transgenic soybean plants are significantly lower than those of non-transgenic plants after incubation with Phytophthora sojae. Overexpression of GmIFR in soybean seed affects isoflavone and glyceollins expression levels, as well as the transcriptional level of multiple genes involved in the phenylpropanal pathway | Glycine max |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.1.45 | cytoplasm | subcellular localization analysis in transgenic protoplasts | Glycine max | 5737 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.45 | 2'-hydroxyformononetin + NADPH + H+ | Glycine max | GmIFR is an isoflavone reductase | vestitone + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.45 | Glycine max | C6TER4 | cv. Suinong10 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.1.45 | hypocotyl | - |
Glycine max | - |
1.3.1.45 | seedling | - |
Glycine max | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.45 | 2'-hydroxyformononetin + NADPH + H+ | - |
Glycine max | vestitone + NADP+ | - |
? | |
1.3.1.45 | 2'-hydroxyformononetin + NADPH + H+ | GmIFR is an isoflavone reductase | Glycine max | vestitone + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.45 | ? | x * 34920, sequence calculation, x * 34000, recombinant enzyme, SDS-PAGE | Glycine max |
1.3.1.45 | More | The predicted three-dimensional model prediction | Glycine max |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.45 | GmIFR | - |
Glycine max |
1.3.1.45 | IFR | - |
Glycine max |
1.3.1.45 | isoflavone reductase | - |
Glycine max |
1.3.1.45 | LOC100786578 | locus name | Glycine max |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.45 | NADP+ | - |
Glycine max |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.3.1.45 | Glycine max | sequence calculation | - |
6.33 |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.3.1.45 | Glycine max | the enzyme expression is significantly induced by infection with Phytophthora sojae, as well as by treatment with ethephon, abscisic acid, and salicylic acid | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.1.45 | evolution | isoflavone reductases (IFRs) are unique to the plant kingdom. The enzyme is a member of the soybean isoflavone reductase gene family GmIF | Glycine max |
1.3.1.45 | physiological function | isoflavonereductase (IFR)is an enzyme involved in the biosynthetic pathway of isoflavonoid phytoalexin in plants. The enzyme is considered to have a crucial role in plant response to various biotic and abiotic environmental stresses. Overexpression of soybean isoflavone reductase (GmIFR) enhances resistance to Phytophthora sojae in soybean. Enzyme GmIFR might play an important role as an antioxidant to reduce ROS in soybean | Glycine max |