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Literature summary extracted from

  • Li, D.; Yewalkar, S.; Bi, X.; Duff, S.; Posarac, D.; Wang, H.; Woodfin, L.; Hehemann, J.; Potter, S.; Nano, F.
    Heterologous expression of LamA gene encoded endo-beta-1,3-glucanase and CO2 fixation by bioengineered Synechococcus sp. PCC 7002 (2017), Front. Environ. Sci. Eng., 11, 9-9 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expressed in Synechococcus sp. PCC 7002 Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Thermotoga maritima Q9WXN1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminarin + H2O
-
Thermotoga maritima ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 29000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 endo-beta-1,3-glucanase
-
Thermotoga maritima
3.2.1.39 LamA
-
Thermotoga maritima
3.2.1.39 laminarinase
-
Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 70
-
-
Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.39 40 90 the recombinant enzyme possesses a stable activity in the range of 40°C to 80°C. At 80°C and 90°C, the enzyme shows 92% and 37% of its maximum activity, respectively Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 80 100 at 80°C and 90°C, the enzyme shows a half-life of 40 min and 20 min, respectively. When the temperature reaches 100°C, the enzyme is rapidly deactivated, with a half-life of less than 5 min Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 8
-
-
Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.39 7 8.5 the enzyme retains 85% of its maximal activity between pH 7.0 and 8.5. There is a sharp decrease in activity below pH 7.0 and the enzyme becomes almost inactive below pH 5.5 Thermotoga maritima