EC Number | Application | Comment | Organism |
---|---|---|---|
1.10.3.1 | food industry | inhibition of enzyme PPO is important in the food industry, due its role in browning | Physalis peruviana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.1 | ascorbate | - |
Physalis peruviana | |
1.10.3.1 | CaCl2 | - |
Physalis peruviana | |
1.10.3.1 | Citric acid | weak inhibition | Physalis peruviana | |
1.10.3.1 | EDTA | - |
Physalis peruviana | |
1.10.3.1 | fukugiside | - |
Physalis peruviana | |
1.10.3.1 | hydroquinone | - |
Physalis peruviana | |
1.10.3.1 | L-cysteine | - |
Physalis peruviana | |
1.10.3.1 | additional information | no inhibition by amentoflavone, BHT, and morelloflavone | Physalis peruviana | |
1.10.3.1 | NaCl | - |
Physalis peruviana | |
1.10.3.1 | quercetin | - |
Physalis peruviana | |
1.10.3.1 | sodium sulfite | - |
Physalis peruviana | |
1.10.3.1 | Tannic acid | - |
Physalis peruviana | |
1.10.3.1 | tartaric acid | - |
Physalis peruviana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.1 | 0.56 | - |
chlorogenic acid | pH 5.0, 20°C | Physalis peruviana | |
1.10.3.1 | 3.88 | - |
4-methylcatechol | pH 5.0, 20°C | Physalis peruviana | |
1.10.3.1 | 203.8 | - |
catechol | pH 5.0, 20°C | Physalis peruviana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.1 | 2 4-methylcatechol + O2 | Physalis peruviana | - |
2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
1.10.3.1 | 2 4-methylcatechol + O2 | Physalis peruviana Colombian ecotype | - |
2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
1.10.3.1 | 2 catechol + O2 | Physalis peruviana | - |
2 1,2-benzoquinone + 2 H2O | - |
? | |
1.10.3.1 | 2 catechol + O2 | Physalis peruviana Colombian ecotype | - |
2 1,2-benzoquinone + 2 H2O | - |
? | |
1.10.3.1 | chlorogenic acid + O2 | Physalis peruviana | best substrate | ? | - |
? | |
1.10.3.1 | chlorogenic acid + O2 | Physalis peruviana Colombian ecotype | best substrate | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.1 | Physalis peruviana | - |
- |
- |
1.10.3.1 | Physalis peruviana Colombian ecotype | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.3.1 | native enzyme 170fold from fruits by acetone and ATPS precipitation | Physalis peruviana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.10.3.1 | fruit | the activity (measured with 4-methylcatechol as substrate) decreases during fruit ripening, overview | Physalis peruviana | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 204.69 | - |
purified enzyme, substrate chlorogenic acid, pH 5.0, 20°C | Physalis peruviana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.1 | 2 4-methylcatechol + O2 | - |
Physalis peruviana | 2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
1.10.3.1 | 2 4-methylcatechol + O2 | - |
Physalis peruviana Colombian ecotype | 2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
1.10.3.1 | 2 catechol + O2 | - |
Physalis peruviana | 2 1,2-benzoquinone + 2 H2O | - |
? | |
1.10.3.1 | 2 catechol + O2 | - |
Physalis peruviana Colombian ecotype | 2 1,2-benzoquinone + 2 H2O | - |
? | |
1.10.3.1 | chlorogenic acid + O2 | best substrate | Physalis peruviana | ? | - |
? | |
1.10.3.1 | chlorogenic acid + O2 | best substrate | Physalis peruviana Colombian ecotype | ? | - |
? | |
1.10.3.1 | additional information | the specificity of PPO toward chlorogenic acid is approximately 7 times greater than that for 4-methylcatechol and 370 times greater than that for catechol. No activity with L-tyrosine. The Cape gooseberry PPO lacks monophenolase (cresolase) activity, EC 1.14.18.1 | Physalis peruviana | ? | - |
? | |
1.10.3.1 | additional information | the specificity of PPO toward chlorogenic acid is approximately 7 times greater than that for 4-methylcatechol and 370 times greater than that for catechol. No activity with L-tyrosine. The Cape gooseberry PPO lacks monophenolase (cresolase) activity, EC 1.14.18.1 | Physalis peruviana Colombian ecotype | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.10.3.1 | polyphenol oxidase | - |
Physalis peruviana |
1.10.3.1 | PPO | - |
Physalis peruviana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 20 | - |
with chlorogenic acid | Physalis peruviana |
1.10.3.1 | 25 | - |
with 4-methylcatechol | Physalis peruviana |
1.10.3.1 | 40 | - |
with catechol | Physalis peruviana |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 20 | 50 | pH profiles withdifferent substrates, overview | Physalis peruviana |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 20.8 | - |
kinetic parameters for the thermal inactivation of Cape gooseberry PPO, overview | Physalis peruviana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 5 | - |
with chlorogenic acid | Physalis peruviana |
1.10.3.1 | 5.5 | - |
with catechol and 4-methylcatechol | Physalis peruviana |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 3.6 | 6.5 | pH profiles with different substrates, overview | Physalis peruviana |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.10.3.1 | 0.002 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | quercetin | |
1.10.3.1 | 0.044 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | L-cysteine | |
1.10.3.1 | 0.044 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | hydroquinone | |
1.10.3.1 | 0.045 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | ascorbate | |
1.10.3.1 | 0.049 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | quercetin | |
1.10.3.1 | 0.052 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | L-cysteine | |
1.10.3.1 | 0.06 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | ascorbate | |
1.10.3.1 | 0.062 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | Tannic acid | |
1.10.3.1 | 0.15 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | sodium sulfite | |
1.10.3.1 | 0.157 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | sodium sulfite | |
1.10.3.1 | 0.372 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | Tannic acid | |
1.10.3.1 | 0.536 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | fukugiside | |
1.10.3.1 | 0.563 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | hydroquinone | |
1.10.3.1 | 0.632 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | fukugiside | |
1.10.3.1 | 3.56 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | CaCl2 | |
1.10.3.1 | 5.01 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | NaCl | |
1.10.3.1 | 30.96 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | CaCl2 | |
1.10.3.1 | 39.54 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | NaCl |