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Literature summary extracted from

  • Bravo, K.; Osorio, E.
    Characterization of polyphenol oxidase from Cape gooseberry (Physalis peruviana L.) fruit (2016), Food Chem., 197, 185-190 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.1 food industry inhibition of enzyme PPO is important in the food industry, due its role in browning Physalis peruviana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 ascorbate
-
Physalis peruviana
1.10.3.1 CaCl2
-
Physalis peruviana
1.10.3.1 Citric acid weak inhibition Physalis peruviana
1.10.3.1 EDTA
-
Physalis peruviana
1.10.3.1 fukugiside
-
Physalis peruviana
1.10.3.1 hydroquinone
-
Physalis peruviana
1.10.3.1 L-cysteine
-
Physalis peruviana
1.10.3.1 additional information no inhibition by amentoflavone, BHT, and morelloflavone Physalis peruviana
1.10.3.1 NaCl
-
Physalis peruviana
1.10.3.1 quercetin
-
Physalis peruviana
1.10.3.1 sodium sulfite
-
Physalis peruviana
1.10.3.1 Tannic acid
-
Physalis peruviana
1.10.3.1 tartaric acid
-
Physalis peruviana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 0.56
-
chlorogenic acid pH 5.0, 20°C Physalis peruviana
1.10.3.1 3.88
-
4-methylcatechol pH 5.0, 20°C Physalis peruviana
1.10.3.1 203.8
-
catechol pH 5.0, 20°C Physalis peruviana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.1 2 4-methylcatechol + O2 Physalis peruviana
-
2 4-methyl-1,2-benzoquinone + 2 H2O
-
?
1.10.3.1 2 4-methylcatechol + O2 Physalis peruviana Colombian ecotype
-
2 4-methyl-1,2-benzoquinone + 2 H2O
-
?
1.10.3.1 2 catechol + O2 Physalis peruviana
-
2 1,2-benzoquinone + 2 H2O
-
?
1.10.3.1 2 catechol + O2 Physalis peruviana Colombian ecotype
-
2 1,2-benzoquinone + 2 H2O
-
?
1.10.3.1 chlorogenic acid + O2 Physalis peruviana best substrate ?
-
?
1.10.3.1 chlorogenic acid + O2 Physalis peruviana Colombian ecotype best substrate ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Physalis peruviana
-
-
-
1.10.3.1 Physalis peruviana Colombian ecotype
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 native enzyme 170fold from fruits by acetone and ATPS precipitation Physalis peruviana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 fruit the activity (measured with 4-methylcatechol as substrate) decreases during fruit ripening, overview Physalis peruviana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 204.69
-
purified enzyme, substrate chlorogenic acid, pH 5.0, 20°C Physalis peruviana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 2 4-methylcatechol + O2
-
Physalis peruviana 2 4-methyl-1,2-benzoquinone + 2 H2O
-
?
1.10.3.1 2 4-methylcatechol + O2
-
Physalis peruviana Colombian ecotype 2 4-methyl-1,2-benzoquinone + 2 H2O
-
?
1.10.3.1 2 catechol + O2
-
Physalis peruviana 2 1,2-benzoquinone + 2 H2O
-
?
1.10.3.1 2 catechol + O2
-
Physalis peruviana Colombian ecotype 2 1,2-benzoquinone + 2 H2O
-
?
1.10.3.1 chlorogenic acid + O2 best substrate Physalis peruviana ?
-
?
1.10.3.1 chlorogenic acid + O2 best substrate Physalis peruviana Colombian ecotype ?
-
?
1.10.3.1 additional information the specificity of PPO toward chlorogenic acid is approximately 7 times greater than that for 4-methylcatechol and 370 times greater than that for catechol. No activity with L-tyrosine. The Cape gooseberry PPO lacks monophenolase (cresolase) activity, EC 1.14.18.1 Physalis peruviana ?
-
?
1.10.3.1 additional information the specificity of PPO toward chlorogenic acid is approximately 7 times greater than that for 4-methylcatechol and 370 times greater than that for catechol. No activity with L-tyrosine. The Cape gooseberry PPO lacks monophenolase (cresolase) activity, EC 1.14.18.1 Physalis peruviana Colombian ecotype ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 polyphenol oxidase
-
Physalis peruviana
1.10.3.1 PPO
-
Physalis peruviana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 20
-
with chlorogenic acid Physalis peruviana
1.10.3.1 25
-
with 4-methylcatechol Physalis peruviana
1.10.3.1 40
-
with catechol Physalis peruviana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.1 20 50 pH profiles withdifferent substrates, overview Physalis peruviana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 20.8
-
kinetic parameters for the thermal inactivation of Cape gooseberry PPO, overview Physalis peruviana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 5
-
with chlorogenic acid Physalis peruviana
1.10.3.1 5.5
-
with catechol and 4-methylcatechol Physalis peruviana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.1 3.6 6.5 pH profiles with different substrates, overview Physalis peruviana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.10.3.1 0.002
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana quercetin
1.10.3.1 0.044
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana L-cysteine
1.10.3.1 0.044
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana hydroquinone
1.10.3.1 0.045
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana ascorbate
1.10.3.1 0.049
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana quercetin
1.10.3.1 0.052
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana L-cysteine
1.10.3.1 0.06
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana ascorbate
1.10.3.1 0.062
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana Tannic acid
1.10.3.1 0.15
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana sodium sulfite
1.10.3.1 0.157
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana sodium sulfite
1.10.3.1 0.372
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana Tannic acid
1.10.3.1 0.536
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana fukugiside
1.10.3.1 0.563
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana hydroquinone
1.10.3.1 0.632
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana fukugiside
1.10.3.1 3.56
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana CaCl2
1.10.3.1 5.01
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana NaCl
1.10.3.1 30.96
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana CaCl2
1.10.3.1 39.54
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana NaCl