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Literature summary extracted from

  • Brinkmann, U.; Reineke, W.
    Degradation of chlorotoluenes by in vivo constructed hybrid strains problems of enzyme specificity, induction and prevention of meta-pathway (1992), FEMS Microbiol. Lett., 96, 81-88 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.15.26 degradation presence of chlorinated toluenes induces expression of enzymes of the xylene degradation sequence. Conjugative transfer of the TOL plasmid from Pseudomonas putida strain PaW1 to Pseudomonas sp. strain B13 and Pseudomonas cepacia strain JH230 allows the isolation of hybrid strains capable of growing in the presence of 3-chloro-, 4-chloro- and 3,5-dichlorotoluene Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.26 Pseudomonas putida
-
-
-
1.14.15.26 Pseudomonas putida PaW1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.26 2-fluorotoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 2-fluorobenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 2-fluorotoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida PaW1 2-fluorobenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 3-chlorotoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 3-chlorobenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 3-chlorotoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida PaW1 3-chlorobenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 3-fluorotoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 3-fluorobenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 3-fluorotoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida PaW1 3-fluorobenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 3-methyltoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 3-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 3-methyltoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida PaW1 3-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 4-chlorotoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-chlorobenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 4-chlorotoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida PaW1 4-chlorobenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 4-fluorotoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-fluorobenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 4-methyltoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.26 toluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida benzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

General Information

EC Number General Information Comment Organism
1.14.15.26 physiological function presence of chlorinated toluenes induces expression of enzymes of the xylene degradation sequence. Conjugative transfer of the TOL plasmid from Pseudomonas putida strain PaW1 to Pseudomonas sp. strain B13 and Pseudomonas cepacia strain JH230 allows the isolation of hybrid strains capable of growing in the presence of 3-chloro-, 4-chloro- and 3,5-dichlorotoluene Pseudomonas putida