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Literature summary extracted from
- Elicitor-induced biosynthesis of psoralens in Ammi majus L. suspension cultures. Microsomal conversion of demethylsuberosin into (+)marmesin and psoralen (1988), Eur. J. Biochem., 171, 369-375 .
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.66 | 0.0103 | - |
demethylsuberosin | pH 7.5, 20°C | Ammi majus |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.66 | microsome | - |
Ammi majus | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.66 | Ammi majus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.66 | cell suspension culture | - |
Ammi majus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.14.66 | -70°C, stable for at least 6 months | Ammi majus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.66 | demethylsuberosin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Ammi majus | (+)-marmesin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.66 | 20 | - |
- |
Ammi majus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.66 | 15 | - |
80% of maximum activity | Ammi majus |
| 1.14.14.66 | 25 | - |
95% of maximum activity | Ammi majus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.66 | 7.5 | - |
broad | Ammi majus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.66 | cytochrome P450 | - |
Ammi majus | |
| 1.14.14.66 | additional information | NADPH-hemoprotein reductase does not accept NADH | Ammi majus |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.14.14.66 | Ammi majus | expression is induced in response to treatment with elicitor preparations from either Alternaria carthami Chowdhury or Phytophthora megasperma f.sp. glycinea | up |
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