EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.62 | gene CYP72C, DNA and amino acid sequence determination and analysis, sequence comparisons, transcriptome analysis, real-time PCR expression analysis | Catharanthus roseus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.19.62 | endoplasmic reticulum | an endoplasmic reticulum-anchored enzyme | Catharanthus roseus | 5783 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.62 | loganin + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | - |
secologanin + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
1.14.19.62 | additional information | Catharanthus roseus | the second secologanin synthase isoform SLS2 produces both secologanin and secoxyloganin | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.62 | Catharanthus roseus | U5NDT8 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.19.62 | additional information | SLS2 might be the prominent isoform of secologanin biosynthesis in the aerial parts of the plant | Catharanthus roseus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.62 | loganin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Catharanthus roseus | secologanin + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
1.14.19.62 | additional information | the second secologanin synthase isoform SLS2 produces both secologanin and secoxyloganin | Catharanthus roseus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.62 | CYP72C | - |
Catharanthus roseus |
1.14.19.62 | SLS | - |
Catharanthus roseus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.19.62 | 30 | - |
assay at | Catharanthus roseus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.19.62 | 7.5 | - |
assay at | Catharanthus roseus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.62 | cytochrome P450 | - |
Catharanthus roseus | |
1.14.19.62 | NADPH-hemoprotein reductase | - |
Catharanthus roseus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.19.62 | metabolism | the enzyme is important in the monoterpenoid indole alkaloid (MIA) biosynthesis in Catharanthus roseus, subcellular organization of the central steps of the pathway, overview. The ring-opening reaction of loganin in the biosynthesis of secologanin is catalyzed by the fourth P450 of this pathway, secologanin synthase (SLS, CYP72A1). Both isozymes SLS1 and SLS2 not only catalyze the oxidative ring cleavage of loganin to produce secologanin but also perform the oxidation of secologanin into secoxyloganin. SLS1 and SLS2 constitute the third type of P450 from the seco-iridoid pathway performing more than one catalytic reaction. Additional enzymes might convert secoxyloganin back to secologanin, the subcellular compartmentation of secologanin biosynthesis may also limit secoxyloganin formation in planta. Secoxyloganin is an acidic compound derived from secologanin that is no longer able to be condensed with tryptamine by STR in the vacuole, due to the absence of the aldehyde function. If secoxyloganin formation occurs in vivo, the resulting depletion of the secologanin pool is deleterious for the subsequent synthesis of MIAs | Catharanthus roseus |
1.14.19.62 | additional information | consensus transcriptome analysis of Catharanthus roseus, detailed overview | Catharanthus roseus |
1.14.19.62 | physiological function | secologanin synthases (SLS) are P450 enzymes that catalyze an unusual ring-opening reaction of loganin in the biosynthesis of the monoterpenoid indole alkaloids (MIA) precursor secologanin | Catharanthus roseus |