EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.9 | gene rebH, functional recombinant expression of Yfp-tagged enzyme in Nicotianan benthamiana leaf cytosol via Agrobacterium tumefaciens tranfection method under control of the CaMV 35S promoter leading to formation of 7-chlorotryptophan, coexpression with gene rebF from Lechevalieria aerocolonigenes, different genetic constructs, overview. Coexpression and concerted activity of tryptophan 6-halogenase Stth, EC 1.14.19.59, and RebH lead to formation of 6,7-halotryptophan | Lentzea aerocolonigenes |
1.14.19.59 | gene stth, functional recombinant expression of Yfp-tagged enzyme in Nicotianan benthamiana leaves via Agrobacterium tumefaciens strain EHA105 tranfection method under control of the CaMV 35S promoter leading to formation of 6-chlorotryptophan, coexpression with gene rebF from Lechevalieria aerocolonigenes, different genetic constructs, overview. Coexpression and concerted activity of Stth and tryptophan 7-halogenase RebH, EC 1.14.19.9, lead to formation of 6,7-halotryptophan | Streptomyces toxytricini |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.9 | 6-chloro-L-tryptophan + FADH2 + chloride + O2 + H+ | Lentzea aerocolonigenes | - |
6,7-dichloro-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.9 | L-tryptophan + FADH2 + chloride + O2 + H+ | Lentzea aerocolonigenes | - |
7-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.59 | 7-chloro-L-tryptophan + FADH2 + chloride + O2 + H+ | Streptomyces toxytricini | - |
6,7-dichloro-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.59 | L-tryptophan + FADH2 + chloride + O2 + H+ | Streptomyces toxytricini | - |
6-chloro-L-tryptophan + FAD + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.9 | Lentzea aerocolonigenes | Q8KHZ8 | - |
- |
1.14.19.59 | Streptomyces toxytricini | E9P162 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.9 | 6-chloro-L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Lentzea aerocolonigenes | 6,7-dichloro-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.9 | L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Lentzea aerocolonigenes | 7-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.59 | 7-chloro-L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces toxytricini | 6,7-dichloro-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.59 | L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces toxytricini | 6-chloro-L-tryptophan + FAD + 2 H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.9 | RebH | - |
Lentzea aerocolonigenes |
1.14.19.59 | sttH | - |
Streptomyces toxytricini |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.9 | FAD | - |
Lentzea aerocolonigenes | |
1.14.19.59 | FAD | - |
Streptomyces toxytricini |