Literature summary extracted from

Gan, R.; Perez, J.G.; Carlson, E.D.; Ntai, I.; Isaacs, F.J.; Kelleher, N.L.; Jewett, M.C.
Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins (2017), Biotechnol. Bioeng., 114, 1074-1086 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
6.1.1.20	expressed in Escherichia coli	Methanocaldococcus jannaschii

Protein Variants

EC Number	Protein Variants	Comment	Organism
6.1.1.20	D286R	the mutant shows improvement of activity over the parental enzyme	Methanocaldococcus jannaschii
6.1.1.20	H283T/P284S/M285D/D286V	the mutant shows approximate 8fold improvement of activity over the parental enzyme	Methanocaldococcus jannaschii
6.1.1.20	P258A/F261P/H283L/M285F/D286Y	the mutant shows improvement of activity over the parental enzyme	Methanocaldococcus jannaschii
6.1.1.20	P284V/D286R	the mutant shows improvement of activity over the parental enzyme	Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6.1.1.20	ATP + 4-azido-L-phenylalanine + tRNAPhe	Methanocaldococcus jannaschii	-	AMP + diphosphate + 4-azido-L-phenylalanyl-tRNAPhe	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.1.1.20	Methanocaldococcus jannaschii	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.1.1.20	ATP + 4-azido-L-phenylalanine + tRNAPhe	-	Methanocaldococcus jannaschii	AMP + diphosphate + 4-azido-L-phenylalanyl-tRNAPhe	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
6.1.1.20	p-azido-phenylalanyl-tRNA synthetase	-	Methanocaldococcus jannaschii
6.1.1.20	pAzFRS	-	Methanocaldococcus jannaschii

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
6.1.1.20	ATP	-	Methanocaldococcus jannaschii

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Release 2023.1 (January 2023)