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Literature summary extracted from
- Cloning and characterization of a novel beta-carotene hydroxylase gene from Lycium barbarum and its expression in Escherichia coli (2014), Biotechnol. Appl. Biochem., 61, 637-645 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.15.24 | expressed in Escherichia coli BL21(DE3) cells | Lycium barbarum |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.15.24 | chloroplast | - |
Lycium barbarum | 9507 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.24 | beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | Lycium barbarum | - |
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.24 | Lycium barbarum | U5Y6R4 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.24 | Ni-NTA column chromatography | Lycium barbarum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.15.24 | flower | - |
Lycium barbarum | - |
| 1.14.15.24 | fruit | highest expression | Lycium barbarum | - |
| 1.14.15.24 | leaf | - |
Lycium barbarum | - |
| 1.14.15.24 | root | - |
Lycium barbarum | - |
| 1.14.15.24 | stem | lowest expression | Lycium barbarum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.24 | beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | - |
Lycium barbarum | zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.15.24 | ? | x * 37000, SDS-PAGE | Lycium barbarum |
| 1.14.15.24 | ? | x * 34800, calculated from amino acid sequence | Lycium barbarum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.15.24 | beta-carotene hydroxylase | - |
Lycium barbarum |
| 1.14.15.24 | Chyb | - |
Lycium barbarum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.14.15.24 | Lycium barbarum | calculated from amino acid sequence | - |
8.4 |
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